國家衛生研究院 NHRI:Item 3990099045/2985
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    题名: Monitoring and quantitative assessment of tumor burden using in vivo bioluminescence imaging
    作者: Chen, CC;Hwang, JJ;Ting, G;Tseng, YL;Wang, SJ;Whang-Peng, J
    贡献者: National Institute of Cancer Research
    摘要: In vivo bioluminescence imaging (BLI) is a sensitive imaging modality that is rapid and accessible, and may comprise an ideal tool for evaluating tumor growth. In this study, the kinetic of tumor growth has been assessed in C26 colon carcinoma bearing BALB/c mouse model. The ability of BLI to noninvasively quantitate the growth of subcutaneous tumors transplanted with C26 cells genetically engineered to stably express firefly luciferase and herpes simplex virus type-1 thymidine kinase (C26/tk-luc). A good correlation (R-2 = 0.998) of photon emission to the cell number was found in vitro. Tumor burden and tumor volume were monitored in vivo over time by quantitation of photon emission using Xenogen IVIS 50 and standard external caliper measurement, respectively. At various time intervals, tumor-bearing mice were imaged to determine the correlation of in vivo BLI to tumor volume. However, a correlation of BLI to tumor volume was observed when tumor volume was smaller than 1000 mm(3) (R-2 = 0.907). gamma Scintigraphy combined with [I-131]FIAU was another imaging modality used for verifying the previous results. In conclusion, this study showed that bioluminescence imaging is a powerful and quantitative tool for the direct assay to monitor tumor growth in vivo. The dual reporter genes transfected tumor-bearing animal model can be applied in the evaluation of the efficacy of new developed anticancer drugs.
    关键词: Instruments & Instrumentation;Nuclear Science & Technology;Physics, Particles & Fields;Spectroscopy
    日期: 2007-02-01
    關聯: Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. 2007 Feb;571(1-2):437-441.
    Link to: http://dx.doi.org/10.1016/j.nima.2006.10.129
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0168-9002&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000244599100106
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33846383672
    显示于类别:[彭汪嘉康(1996-2007)] 會議論文/會議摘要

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