國家衛生研究院 NHRI:Item 3990099045/2925

NHRI > National Institute of Cancer Research > Ming Jacqueline Liu(1996-2007) > Periodical Articles > Item 3990099045/2925

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Title:	In utero transplantation of human bone marrow-derived multipotent mesenchymal stem cells in mice
Authors:	Chou, SH;Kuo, TK;Liu, M;Lee, OK
Contributors:	National Institute of Cancer Research
Abstract:	Mesenchymal stem cells (MSCs) are multipotent cells that can be isolated from human bone marrow and possess the potential to differentiate into progenies of embryonic mesoderm. However, current evidence is based predominantly on in vitro experiments. We used a murine model of in utero transplantation (IUT) to study the engraftment capabilities of human MSCs. MSCs were obtained from bone marrow by negative immunoselection and limiting dilution, and were characterized by flow cytometry and by in vitro differentiation into osteoblasts, chondrocytes, and adipocytes. MSCs were transplanted into fetal mice at a gestational age of 14 days. Engraftment of human MSCs was determined by flow cytometry, polymerase chain reaction, and fluorescence in situ hybridization (FISH). MSCs engrafted into tissues originating from all three germ layers and persisted for up to 4 months or more after delivery, as evidenced by the expression of the human-specific beta-2 microglobulin gene and by FISH for donor-derived cells. Donor-derived CD45(+) cells were detectable in the peripheral blood of recipients, suggesting the participation of MSCs in hematopoiesis at the fetal stage. This model can further serve to evaluate possible applications of MSCs.
Keywords:	Orthopedics
Date:	2006-03
Relation:	Journal of Orthopaedic Research. 2006 Mar;24(3):301-312.
Link to:	http://dx.doi.org/10.1002/jor.20047
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0736-0266&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000235919900001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33645742401
Appears in Collections:	[Ming Jacqueline Liu(1996-2007)] Periodical Articles

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