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http://ir.nhri.org.tw/handle/3990099045/2913
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| Title: | IFN-beta induces caspase-mediated apoptosis by disrupting mitochondria in human advanced stage colon cancer cell lines |
| Authors: | Juang, SH;Wei, SJ;Hung, YM;Hsu, CY;Yang, DM;Liu, KJ;Chen, WS;Yang, WK |
| Contributors: | National Institute of Cancer Research |
| Abstract: | Various human colon cancer cell lines tested in vitro differed significantly in susceptibility to growth inhibition of recombinant human interferon-beta (rHuIFN-beta). Two p53-mutant lines, COH and CC-M2, derived from high-grade colon adenocarcinoma, showed signs of apoptosis after treatment with 250 IU/ml of HuIFN-beta in the culture medium. The similarly p53-mutated HT-29 line from a grade I adenocarcinoma showed no apoptosis, however, and only cell cycle G(1)/G(0) or S phase retardation with 1000 IU/ml HuIFN-beta. After HuIFN-beta exposure, COH and CC-M2 cells showed increased levels of Fas and FasL proteins, alteration of mitochondrial membrane potential, and activation of caspase-9, caspase-8, and caspase-3 in a time-dependent manner. Treatment of COH and CC-M2 cells with anti-FasL antibodies or rFas/Fc fusion protein, however, could not prevent the apoptosis induced by HuIFN-beta. In contrast, cell-permeable specific inhibitors of the three caspases could inhibit the DNA fragmentation and cell death but not the mitochondrial membrane potential changes. Treatment with mitochondria-stabilizing reagents could significantly abrogate the apoptosis and caspase activation induced by HuIFN-beta. These results suggest that in COH and CC-M2 colon cancer cell lines, HuIFN-beta induces apoptosis mainly through mitochondrial membrane alteration and subsequent activation of the caspase cascade pathway, but not by the Fas/FasL interaction or the p53-dependent apoptotic mechanism. |
| Keywords: | Biochemistry & Molecular Biology;Cell Biology;Immunology |
| Date: | 2004-04 |
| Relation: | Journal of Interferon and Cytokine Research. 2004 Apr;24(4):231-243. |
| Link to: | http://dx.doi.org/10.1089/107999004323034105 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1079-9907&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000221057700003 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=1942420886 |
| Appears in Collections: | [劉柯俊] 期刊論文
[莊聲宏(1997-2004)] 期刊論文
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| 000221057700003.pdf | | 609Kb | Adobe PDF | 850 | View/Open |
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