國家衛生研究院 NHRI:Item 3990099045/2910

NHRI > National Institute of Cancer Research > Ming Jacqueline Liu(1996-2007) > Periodical Articles > Item 3990099045/2910

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2910

Title:	Colon cancer cells with high invasive potential are susceptible to induction of apoptosis by a selective COX-2 inhibitor
Authors:	Chen, WS;Liu, JH;Wei, SJ;Liu, JM;Hong, CY;Yang, WK
Contributors:	National Institute of Cancer Research
Abstract:	Cyclooxygenase-2 (COX-2) expression has been shown to correlate with the invasiveness of colon cancer cells. To further investigate this positive correlation and its possible therapeutic implications, a selective COX-2 inhibitor, etodolac, was tested on three variants of HT-29 colon cancer cell lines, HT-29/Inv1, HT-29/Inv2 and HT-29/Inv3, with graded increases of in vitro Matrigel invasive potential and COX-2 expression levels. HT-29 variants with higher invasive potential were found to be more sensitive to etodolac by in vitro growth inhibition assays, the estimated LD50 being 0.5 mM for highly invasive HT-29/Inv2 and HT-29/Inv3 cells, 0.6 mM for slightly less invasive HT-29/Inv1, and 1.8 mM for the parental HT-29. Treatment of the highly invasive HT-29/Inv2 and Inv3 variants with as little as 0.1 mM etodolac in the growth medium produced signs of apoptosis, as detected by DNA fragmentation and TUNEL (terminal deoxynucleotidyl transferase dUTP-biotin nick end labeling) assay. In vivo experiments in SCID mice showed that etolodac inhibited the growth of subcutaneous tumors induced by HT-29/Inv3 cells significantly more than those by the parental HT-29 cells. These results suggest that COX-2 inhibitors have a potential role in prevention of tumor invasion in colon cancer patients.
Keywords:	Oncology
Date:	2003-03
Relation:	Cancer Science. 2003 Mar;94(3):253-258.
Link to:	http://dx.doi.org/10.1111/j.1349-7006.2003.tb01429.x
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1347-9032&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000182406400006
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0038297351
Appears in Collections:	[Ming Jacqueline Liu(1996-2007)] Periodical Articles

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