國家衛生研究院 NHRI:Item 3990099045/2850
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2850


    Title: Use of bacterially expressed EBNA-1 protein cloned from a nasopharyngeal carcinoma (NPC) biopsy as a screening test for NPC patients
    Authors: Chen, MR;Liu, MY;Hsu, SM;Fong, CC;Chen, CJ;Chen, IH;Hsu, MM;Yang, CS;Chen, JY
    Contributors: National Institute of Cancer Research
    Abstract: EBV serological tests have been used for many years as accessory diagnostic predictors of nasopharyngeal carcinoma (NPC). To increase the sensitivity and specificity of the NPC detection rate, a novel enzyme-linked immunosorbent assay (ELISA) was established using a bacterially-expressed GST-EBNA-1 protein, containing the EBNA-1 sequence cloned from an NPC patient. Serum samples were collected from age- and gender-matched patients with NPC, community control subjects and hospital control patients and tested using this ELISA. The positivity rates were 78.7% (247/314) in NPC, 11.5% (28/244) in hospital controls and 3.8% (10/263) in the community control group. These serum samples were also tested for IgA anti-VCA antibodies and their ability to neutralize EBV DNase and the sensitivities of the anti-VCA antibody and DNase-neutralization tests also were analyzed. The optimum combination is VCA plus EBNA-1, which can identify 92.5% (287/310) of NPC patients, and shows a specificity of 92.7% (242/261) for normal individuals. J. Med. Virol. 64:51-57, 2001. (C) 2001 Wiley-Liss, Inc.
    Keywords: Virology
    Date: 2001-05
    Relation: Journal of Medical Virology. 2001 May;64(1):51-57.
    Link to: http://dx.doi.org/10.1002/jmv.1017
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0146-6615&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000167808900009
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035085293
    Appears in Collections:[Jen-Yang Chen] Periodical Articles

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