國家衛生研究院 NHRI:Item 3990099045/2696
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 12145/12927 (94%)
Visitors : 910195      Online Users : 810
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2696


    Title: Identification of alpha-enolase as an autoantigen in lung cancer: Its overexpression is associated with clinical outcomes
    Authors: Chang, GC;Liu, KJ;Hsieh, CL;Hu, TS;Charoenfuprasert, S;Liu, HK;Luh, KT;Hsu, LH;Wu, CW;Ting, CC;Chen, CY;Chen, KC;Yang, TY;Chou, TY;Wang, WH;Whang-Peng, J;Shih, NY
    Contributors: National Institute of Cancer Research
    Abstract: Purpose: Although existence of humoral immunity has been previously shown in malignant pleural effusions, only a limited number of immunogenic tumor-associated antigens (TAA) have been identified and associated with lung cancer. In this study, we intended to identify more TAAs in pleural effusion-derived tumor cells. Experimental Design: Using morphologically normal lung tissues as a control lysate in Western blotting analyses, 54 tumor samples were screened with autologous effusion antibodies. Biochemical purification and mass spectrometric identification of TAAs were done using established effusion tumor cell lines as antigen sources. We identified a p48 antigen as of-enolase (ENO1). Semiquantitative immunohistochemistry was used to evaluate expression status of ENO1 in the tissue samples of 80 patients with non-small cell lung cancer (NSCLC) and then correlated with clinical variables. Results: Using ENO1-specifc antiserum, up-regulation of ENO1 expression in effusion tumor cells from 11 of 17 patients was clearly observed compared with human normal lung primary epithelial and non-cancer-associated effusion cells. Immunohistochemical studies consistently showed high level of ENO1 expression in all the tumors we have examined thus far. Log-rank and Cox's analyses of ENO1 expression status revealed that its expression level in primary tumors was a key factor contributing to overall- and progression-free survivals of patients (P < 0.05). The same result was also obtained in the early stage of NSCLC patients, showing that tumors expressing relatively higher ENO1 level were tightly correlated with poorer survival outcomes. Conclusions: Our data strongly support a prognostic role of ENO1 in determining tumor malignancy of patients with NSCLC.
    Keywords: Oncology
    Date: 2006-10-01
    Relation: Clinical Cancer Research. 2006 Oct;12(19):5746-5754.
    Link to: http://dx.doi.org/10.1158/1078-0432.CCR-06-0324
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1078-0432&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000241098100022
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33750285970
    Appears in Collections:[Neng-Yao Shih] Periodical Articles
    [Chou-Chik George Ting(2000-2004)] Periodical Articles
    [Ko-Jiunn Liu] Periodical Articles
    [Jacqueline Whang-Peng(1996-2007)] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    000241098100022.pdf436KbAdobe PDF985View/Open


    All items in NHRI are protected by copyright, with all rights reserved.

    Related Items in TAIR

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback