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http://ir.nhri.org.tw/handle/3990099045/2684
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| Title: | Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells |
| Authors: | Shih, JY;Yang, SC;Hong, TM;Yuan, A;Chen, JJW;Yu, CJ;Chang, YL;Lee, YC;Peck, K;Wu, CW;Yang, PC |
| Contributors: | National Institute of Cancer Research |
| Abstract: | Background: Numerous genetic changes are associated with metastasis and invasion of cancer cells. To identify differentially expressed invasion-associated genes, we screened a panel of lung cancer cell lines (CL1-0, CL1-1, CL1-5, and CL1-5-F-4 in order of increasing invasive activity) for such genes and selected one gene, collapsin response mediator protein-1 (CRMP-1), to characterize. Methods: We used a microarray containing 9600 gene sequences to assess gene expression in the cell panel and selected the differentially expressed CRMP-1 gene for further study. We confirmed the differential expression of CRMP-1 with northern and western blot analyses. After transfecting and overexpressing CRMP-1 in highly invasive CL1-5 cells, the cells were assessed morphologically and with an in vitro invasion assay. We used enhanced green fluorescent protein-tagged CRMP-1 and fluorescence microscopy to localize CRMP-1 intracellularly. CRMP-1 expression in 80 lung cancer specimens was determined by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All statistical tests were two-sided. Results: Expression of CRMP-1 was inversely associated with invasive activity in the cell panel, an observation confirmed by northern and western blot analyses. CRMP-1-transfected. CL1-5 cells became rounded and had fewer filopodia and statistically significantly lower in vitro invasive activity than untransfected cells (all P<.001). During interphase, CRMP-1 protein was present uniformly throughout the cytoplasm and sometimes in the nucleus; during mitosis, CRMP-1 was associated with mitotic spindles, centrosomes, and the midbody (in late telophase). Real-time RT-PCR of lung cancer specimens showed that reduced expression of CRMP-1 was statistically significantly associated with advanced disease (stage III or IV; P =.010), lymph node metastasis (N1, N2, and N3; P = .043), early postoperative relapse (P = .030), and shorter survival (P =.016). Conclusions: CRMP-1 appears to be involved in cancer invasion and metastasis and may be an invasion-suppressor gene. |
| Keywords: | Oncology |
| Date: | 2001-09-19 |
| Relation: | Journal of the National Cancer Institute. 2001 Sep;93(18):1392-1400. |
| Link to: | http://dx.doi.org/10.1093/jnci/93.18.1392 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0027-8874&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000171033300010 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035913647 |
| Appears in Collections: | [吳成文(1996-2008)] 期刊論文
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| 000171033300010.pdf | | 173Kb | Adobe PDF | 695 | View/Open |
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