國家衛生研究院 NHRI:Item 3990099045/2611
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    題名: Using siRNA technique to generate transgenic animals with spatiotemporal and conditional gene knockdown
    作者: Chang, HS;Lin, CH;Chen, YC;Yu, WCY
    貢獻者: National Institute of Cancer Research
    摘要: Based on the RNAi technique, we have developed a new approach that generates transgenic animals capable of mimicking human genetic diseases. The new system is a combination of siRNA with Cre-loxP and tetracycline-on. it has the characteristics of being stable, inheritable, and inducible, with the siRNA able to be transcribed tissue specifically. To support the ability of this new method to generate a model for a disease, we created an ABCA1-deficient mouse line that mimics Tangier disease under controlled conditions. Thus, it should now be possible to rapidly establish human genetic diseases as a whole animal model without the use of embryonic stem cell and gene targeting. This system also provides a tool for pathological and pharmacological studies of aspects peculiar to particular human genetic diseases.
    關鍵詞: Pathology
    日期: 2004-11
    關聯: American Journal of Pathology. 2004 Nov;165(5):1535-1541.
    Link to: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1618682
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0002-9440&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000224736800009
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=7244219858
    顯示於類別:[于重元] 期刊論文

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