國家衛生研究院 NHRI:Item 3990099045/2537

NHRI > National Institute of Environmental Health Sciences > Ying-Chin Ko(2003-2009) > Periodical Articles > Item 3990099045/2537

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2537

Title:	Characterization of arecoline-induced effects on cytotoxicity in normal human gingival fibroblasts by global gene expression profiling
Authors:	Chiang, SL;Jiang, SS;Wang, YJ;Chiang, HC;Chen, PH;Tu, HP;Ho, KY;Tsai, YS;Chang, IS;Ko, YC
Contributors:	Division of Environmental Health and Occupational Medicine;National Institute of Cancer Research
Abstract:	Areca nut is the most widely used psychoactive substance and an important environmental risk factor for development of oral premalignant lesions and cancer. Arecoline, the major alkaloid of areca nut, has been known to cause cytotoxicity and genotoxicity in mammalian cells in vivo and in vitro and even contributes to carcinogenicity. However, the susceptible genes accounting for arecoline-induced damage in normal human oral cells are still lacking, which possibly involves in initial molecular damage via alternation of gene expression level on biological pathways. The present study was undertaken to characterize the toxic effects of arecoline in gene expression profiling on normal human gingival fibroblasts (HGF) using cDNA microarray and quantitative realtime reverse transcription PCR. The cytotoxicity of arecoline on HGF-1 cell line was elevated in a dose-dependent manner ( p < 0.05) accompanied with distinct morphological change and formation of intracellular vacuoles were observed. At optimum concentration of arecoline determined from dose-response curve of the cytotoxicity, a large number of genes were significantly repressed than induced by arecoline in global gene expression profiling. Five induced-and seven repressed genes including glutathione synthetase were further validated, and their gene expression changes were increased in a dose-dependent manner in a concentration range of 50-150 mu g/ml. In conclusion, we proposed a tentative model to explain arecoline-induced effects on contribution of oral pathogenesis. The findings identified that 12 susceptible genes can potentially serve as biomarkers of arecoline-induced damage in betel chewers.
Keywords:	Toxicology
Date:	2007-11
Relation:	Toxicological Sciences. 2007 Nov;100(1):66-74.
Link to:	http://dx.doi.org/10.1093/toxsci/kfm201
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1096-6080&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000250686600010
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=35448986653
Appears in Collections:	[Ying-Chin Ko(2003-2009)] Periodical Articles [I-Shou Chang] Periodical Articles [Shih-Sheng Jiang] Periodical Articles

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