國家衛生研究院 NHRI:Item 3990099045/2499
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2499


    Title: Modulation of the arsenic effects on cytotoxicity, viability, and cell cycle in porcine endothelial cells by selenium
    Authors: Yeh, JY;Cheng, LC;Liang, YC;Ou, BR
    Contributors: Division of Environmental Health and Occupational Medicine
    Abstract: The differential effects of arsenic compounds and the effect of selenium on arsenic-induced changes in cytotoxicity, viability, and cell cycle of porcine aorta endothelial cells (PAECs) were investigated. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay indicated that arsenic trioxide (As2O3) and sodium arsenite (NaAsO2) showed similar cytotoxicity, whereas sodium arsenate ( Na2HAsO4) did not show cytotoxicity in PAECs. As2O3 and NaAsO2 at 20 muM decreased PAEC viability, decreased G0/G1 phase, and increased apoptosis. An increased G2/M phase was observed in NaAsO2-treated PAECs, whereas an increase in secondary necrosis ( late apoptosis) was observed in As2O3-treated PAECs. As2O3-induced apoptosis was associated with upregulation of p53 and caspase 3, whereas NaAsO2-induced apoptosis was associated with p53 upregulation. Sodium selenite (Na2SeO3) at 1 nM reduced 20 muM As2O3-induced cytotoxicity, but not apoptosis, at 24 h. Increased glutathione peroxidase (GPX) activity by Na2SeO3 pretreatment in 20 muM As2O3-treated PAECs suggests that Na2SeO3 modulates As2O3-induced cytoxicity by GPX modulation.
    Keywords: Cell Biology;Peripheral Vascular Disease
    Date: 2003-05
    Relation: Endothelium. 2003 May;10(3):127-139.
    Link to: http://dx.doi.org/10.1080/10623320390233391
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000186155800001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0142120473
    Appears in Collections:[Jan-Ying Yeh(2000-2003)] Periodical Articles

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