國家衛生研究院 NHRI:Item 3990099045/2460

NHRI > National Institute of Environmental Health Sciences > Yu-Chen Chang(2000-2003) > Periodical Articles > Item 3990099045/2460

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Title:	Striatal formation of 6-hydroxydopamine in mice treated with pargyline, pyrogallol and methamphetamine
Authors:	Liao, PC;Kuo, YM;Chang, YC;Lin, C;Cherng, CFG;Yu, L
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	Formation of 6-hydroxydopamine (6-OHDA) has been posited in the striatum following methamphetamine treatment and plays a critical role in methamphetamine-induced nigrostriatal dopaminergic toxicity. We used high performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) to determine the formation of 6-OHDA by the treatments of methamphetamine combined with pargyline, a monoamine oxidase inhibitor, and pyrogallol, a catechol-O-methyl-transferase inhibitor, in female C57BL/6J mouse striatum. A substantial amount of 6-OHDA (9.9 +/- 0.7pg/mg wet tissue) was detected in mice treated with pargyline (100 mg/kg) and pyrogallol (25 mg/kg) in combination. Greater striatal 6-OHDA levels were observed in mice treated with combined pargyline, pyrogallol and methamphetamine (50 mg/kg) as compared to mice treated with combined pargyline and pyrogallol. However, mice treated with pargyline and pyragollol in combination followed by one and two doses of methamphetamine exhibited comparable striatal 6-OHDA levels (23.2 +/- 4.3, 27.3 +/- 1.3 pg/mg wet tissue) in our protocol. We conclude that blockade of the primary metabolic pathways of dopamine by inhibiting both monoamine oxidase and catechol-O-methyl-transferase activities is sufficient to induce 6-OHDA formation in the striatum. Acute 6-OHDA accumulation in the striatum can be potentiated by methamphetamine, a potent dopamine releaser, administration following such metabolic inhibitions.
Keywords:	Clinical Neurology;Neurosciences
Date:	2003-05
Relation:	Journal of Neural Transmission. 2003 May;110(5):487-494.
Link to:	http://dx.doi.org/10.1007/s00702-002-0829-x
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0300-9564&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000182715400004
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037734336
Appears in Collections:	[Yu-Chen Chang(2000-2003)] Periodical Articles

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