國家衛生研究院 NHRI:Item 3990099045/2415
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    题名: Simultaneous analysis of urinary 4,4 '-methylenebis(2-chloroaniline) and N-acetyl 4,4 '-methylenebis(2-chloroaniline) using solid-phase extraction and liquid-chromatography/tandem-mass-spectrometry
    作者: Shih, WC;Chen, MF;Huang, CCJ;Uang, SN;Shih, TS;Liou, SH;Wu, KY
    贡献者: Division of Environmental Health and Occupational Medicine
    摘要: Analysis of 4,4'-methylenebis(2-cholroaniline) (MOCA) or its metabolites in urine has been considered as the appropriate method to assess MOCA exposures through inhalation and skin absorption. MOCA and its metabolite, N-acetyl 4,4'-methylenebis(2-chloroaniline) (acetyl-MOCA), are analyzed using methods either limited by sensitivity or sample preparation. Therefore, a solid-phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed to simultaneously analyze MOCA and acetyl-MOCA in urine to serve as biomarkers for MOCA exposure. Protein was precipitated by using acetonitrile, and SPE were applied to clean up samples to eliminate the matrix effect and to improve the recovery. The limit of quantitation of this method was at 1.0 ng/mL for MOCA and 0.03 ng/mL for acetyl-MOCA (signal-to-noise (S/N) ratio = 10). Urinary MOCA and acetyl-MOCA levels in MOCA-exposed workers were analyzed and quantitated to be 191.9 +/- 373.2 (mean standard deviation (SD)) and 11.79 +/- 23.8 ng/mL (N = 54) with the median values 38.6 and 1.8 ng/mL, respectively. MOCA concentrations are significantly correlated with their corresponding acetyl-MOCA levels in urine (Spearman correlation coefficient r = 0.916, p < 0.001). These results show that this method has been successfully developed and provides high-throughput potential to analyze MOCA and acetyl-MOCA to serve as exposure biomarkers for future study of the potential health effects associated with MOCA exposures.
    关键词: Chemistry, Analytical;Spectroscopy
    日期: 2007
    關聯: Rapid Communications in Mass Spectrometry. 2007;21(24):4073-4078.
    Link to: http://dx.doi.org/10.1002/rcm.3314
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0951-4198&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000251833200019
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=37649000912
    显示于类别:[吳焜裕(2001-2008)] 期刊論文

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