國家衛生研究院 NHRI:Item 3990099045/2265

NHRI > Institute of Molecular and Genomic Medicine > Chi-Ying F. Huang(1998-2005) > Periodical Articles > Item 3990099045/2265

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2265

Title:	Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma
Authors:	Tsou, AP;Yang, CW;Huang, CYF;Yu, RCT;Lee, YCG;Chang, CW;Chen, BR;Chung, YF;Fann, MJ;Chi, CW;Chiu, JH;Chou, CK
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G(2)/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.
Keywords:	Biochemistry & Molecular Biology;Oncology;Cell Biology;Genetics & Heredity
Date:	2003-01-16
Relation:	Oncogene. 2003 Jan;22(2):298-307.
Link to:	http://dx.doi.org/10.1038/sj.onc.1206129
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0950-9232&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000180322400015
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037448686
Appears in Collections:	[Chi-Ying F. Huang(1998-2005)] Periodical Articles

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