國家衛生研究院 NHRI:Item 3990099045/2260

NHRI > Institute of Molecular and Genomic Medicine > Chi-Ying F. Huang(1998-2005) > Periodical Articles > Item 3990099045/2260

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2260

Title:	M phase phosphorylation of cytoplasmic dynein intermediate chain and p150(Glued)
Authors:	Huang, CYF;Chang, CPB;Huang, CL;Ferrell, JE
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	To understand how the dramatic cell biological changes of oocyte maturation are brought about, we have begun to identify proteins whose phosphorylation state changes during Xenopus oocyte maturation. Here we have focused on one such protein, p83. We partially purified p83, obtained peptide sequence, and identified it as the intermediate chain of cytoplasmic dynein. During oocyte maturation, dynein intermediate chain became hyperphosphorylated at the time of germinal vesicle breakdown and remained hyperphosphorylated throughout the rest of meiosis and early embryogenesis. p150(Glued), a subunit of dynactin that has been shown to bind to dynein intermediate chain, underwent similar changes in its phosphorylation, Both dynein intermediate chain and p150(Glued) also became hyperphosphorylated during M phase in XTC-2 cells and HeLa cells. Thus, two components of the dynein-dynactin complex undergo coordinated phosphorylation changes at two G(2)/M transitions (maturation in oocytes and mitosis in cells in culture) but remain constitutively in their M phase forms during early embryogenesis. Dynein intermediate chain and p150(Glued) phosphorylation may positively regulate mitotic processes, such as spindle assembly or orientation, or negatively regulate interphase processes such as minus-end-directed organelle trafficking.
Keywords:	Biochemistry & Molecular Biology
Date:	1999-05-14
Relation:	Journal of Biological Chemistry. 1999 May;274(20):14262-14269.
Link to:	http://dx.doi.org/10.1074/jbc.274.20.14262
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1083-351X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000080322200076
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033553411
Appears in Collections:	[Chi-Ying F. Huang(1998-2005)] Periodical Articles

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