國家衛生研究院 NHRI:Item 3990099045/2259

NHRI > Institute of Molecular and Genomic Medicine > Chi-Ying F. Huang(1998-2005) > Periodical Articles > Item 3990099045/2259

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2259

Title:	Global gene expression profiling of dimethylnitrosamine-induced liver fibrosis: From pathological and biochemical data to microarray analysis
Authors:	Su, LJ;Hsu, SL;Yang, JS;Tseng, HH;Huang, SF;Huang, CYF
Contributors:	National Institute of Cancer Research;Division of Molecular and Genomic Medicine
Abstract:	The development of hepatocellular carcinoma (HCC) is generally preceded by cirrhosis, which occurs at the end stage of fibrosis. This is a common and potentially lethal problem of chronic liver disease in Asia. The development of microarrays permits us to monitor transcriptomes on a genome-wide scale; this has dramatically speeded up a comprehensive understanding of the disease process. Here we used dimethylnitrosamine (DMN), a nongenotoxic hepatotoxin, to induce rat necroinflammatory and hepatic fibrosis. During the 6-week time course, histopathological, biochemical, and quantitative RT-PCR analyses confirmed the incidence of necroinflammatory and hepatic fibrosis in this established rat model system. Using the Affymetrix microarray chip, 256 differentially expressed genes were identified from the liver injury samples. Hierarchical clustering of gene expression using a gene ontology database allowed the identification of several stage-specific characters and functionally related clusters that encode proteins related to metabolism, cell growth/maintenance, and response to external challenge. Among these genes, we classified 44 potential necroinflammatory-related genes and 62 potential fibrosis-related markers or drug targets based on histopathological scores. We also compared the results with other data on well-known markers and various other microarray datasets that are available. In conclusion, we believe that the molecular picture of necroinflammatory and hepatic fibrosis from this study may provide novel biological insights into the development of early liver damage molecular classifiers than can be used for basic research and in clinical applications.
Keywords:	Biotechnology & Applied Microbiology;Genetics & Heredity
Date:	2006
Relation:	Gene Expression. 2006;13(2):107-132.
Link to:	http://www.ingentaconnect.com/content/cog/ge/2006/00000013/00000002/art00005
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000240637400005
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33749337898
Appears in Collections:	[Chi-Ying F. Huang(1998-2005)] Periodical Articles [Shiu-Feng Kathy Huang] Periodical Articles

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