國家衛生研究院 NHRI:Item 3990099045/2249

NHRI > Institute of Molecular and Genomic Medicine > Chi-Ying F. Huang(1998-2005) > Periodical Articles > Item 3990099045/2249

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2249

Title:	Phosphorylation and stabilization of HURP by aurora-A: Implication of HURP as a transforming target of aurora-A
Authors:	Yu, CTR;Hsu, JM;Lee, YCG;Tsou, AP;Chou, CK;Huang, CYF
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	Aurora-A, a mitotic serine/threonine kinase with oncogene characteristics, has recently drawn intense attention because of its association with the development of human cancers and its relationship with mitotic progression. Using the gene expression profiles of Aurora-A as a template to search for and compare transcriptome expression profiles in publicly accessible microarray data sets, we identified HURP (encodes hepatoma upregulated protein) as one of the best Aurora-A-correlated genes. Empirical validation indicates that HURP has several characteristics in common with Aurora-A. These two genes have similar expression patterns in hepatocellular carcinoma, liver regeneration after partial hepatectomy, and cell cycle progression and across a variety of tissues and cell lines. Moreover, Aurora-A phosphorylated HURP in vitro and in vivo. Ectopic expression of either the catalytically inactive form of Aurora-A or the HURP-4P mutant, in which the Aurora-A phosphorylation sites were replaced with Ala, resulted in HURP instability and complex disassembly. In addition, HURP-wild-type stable transfectants were capable of growing in low-serum environments whereas HURP-4P grew poorly under low-serum conditions and failed to proliferate. These studies together support the view that the ability to integrate evidence derived from microarray studies into biochemical analyses may ultimately augment our predictive power when analyzing the potential role of poorly characterized proteins. While this combined approach,was simply an initial attempt to answer a range of complex biological questions, our findings do suggest that HURP is a potential oncogenic target of Aurora-A.
Keywords:	Biochemistry & Molecular Biology;Cell Biology
Date:	2005-07
Relation:	Molecular and Cellular Biology. 2005 Jul;25(14):5789-5800.
Link to:	http://dx.doi.org/10.1128/MCB.25.14.5789-5800.2005
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0270-7306&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000230267000001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=21744453954
Appears in Collections:	[Chi-Ying F. Huang(1998-2005)] Periodical Articles

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