國家衛生研究院 NHRI:Item 3990099045/2210
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    题名: Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction
    作者: Jiang, SS;Chen, TC;Yang, JY;Hsiung, CA;Su, IJ;Liu, YL;Chen, PC;Juang, JL
    贡献者: Division of Molecular and Genomic Medicine;Division of Biostatistics and Bioinformatics
    摘要: A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.
    关键词: Immunology;Industrial Relations & Labor;Infectious Diseases;Microbiology
    日期: 2004-01-15
    關聯: Clinical Infectious Diseases. 2004 Jan;38(2):293-296.
    Link to: http://dx.doi.org/10.1086/380841
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1058-4838&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000187568500017
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=1642538325
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