國家衛生研究院 NHRI:Item 3990099045/2182
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2182


    Title: A novel regulator inhibits HBV gene expression
    Authors: Liou, JY;Jeng, KS;Lin, CG;Hu, CP;Chang, CM
    Contributors: Division of Molecular and Genomic Medicine
    Abstract: This study investigated the expression of HBV genes. In the investigation, a novel phenomenon was found in that the expression of HBV genes on a cloned HBV DNA was inhibited by cotransfection with plasmids containing HBV surface genes. Furthermore, the production of viral particles was also reduced as measured by endogenous DNA polymerase assay. S1 mapping analysis and nuclear run on experiment showed that the inhibition occurred at the RNA level due to a decrease in transcriptional initiation. Both mutational analyses and cycloheximide blockage revealed that the HBV surface proteins were not required for inhibition. The deletion study showed that the nucleotides 129 to 620 on the HBV surface gene sequence and the donor and acceptor site (SD/SA) sequence of SV40 were both required for inhibition activity. Furthermore, there was no anti-sense HBV RNA in the transfected cells. Based on these results, a sense RNA of HBV was proposed as a novel regulator which can inhibit HBV gene expression.
    Keywords: Medicine, Research & Experimental
    Date: 1998-09
    Relation: Journal of Biomedical Science. 1998 Sep-Oct;5(5):343-354.
    Link to: http://dx.doi.org/10.1007/BF02253444
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000076373800004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031717784
    Appears in Collections:[Chung-Ming Chang] Periodical Articles
    [King-Song Jeng(1997-2002)] Periodical Articles

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