國家衛生研究院 NHRI:Item 3990099045/2139

NHRI > Institute of Molecular and Genomic Medicine > Yuh-Shan Jou(1996-2005) > Periodical Articles > Item 3990099045/2139

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2139

Title:	Diverse cellular transformation capability of overexpressed genes in human hepatocellular carcinoma
Authors:	Huang, JS;Chao, CC;Su, TL;Yeh, SH;Chen, DS;Chen, CT;Chen, PJ;Jou, YS
Contributors:	Division of Molecular and Genomic Medicine;Division of Biotechnology and Pharmaceutical Research
Abstract:	For isolation of novel cellular transforming genes that potentially participated in hepatocarcinogenesis, we conducted anchorage-independent growth (AIG) assays on 10 human liver cancer cell lines and observed strong AIG capabilities in PLC5 and Huh7 but negligible in Tong cells. After cloning of genes by differential subtractive chain reactions (DSC) from strong AIG to AIG negative cells, we sequenced 2304 clones and identified 245 genes. After four stringent criteria for selection of transforming genes among DSC clones, our results of quantitative RT-PCR analysis indicated that six genes, DDX3, EIF3S2, CLIC1, HDGF, GPC3, and HSPCA were overexpressed in 64%, 62%, 60%, 58%, 49%, and 47%, respectively, of 45 hepatocellular carcinoma (HCC) tissues. The results of cellular transformation capability by AIG assays indicated that the transfectants of EIF3S2 showed the strongest (>100-fold), DDX3 and CLIC1 were moderate, GPC3 and HSPCA were weak, and HDGF was none in forming colonies in soft agar. Together, our results suggested that Tong is a suitable human cell line for screening of overexpressed and/or cellular transforming genes. In addition, our results suggested that diverse functions of cellular transforming genes in various biological pathways could transform human Tong cells and potentially reveal new targets for drug development of HCC. (C) 2004 Elsevier Inc. All rights reserved.
Keywords:	Biochemistry & Molecular Biology;Biophysics
Date:	2004-03-19
Relation:	Biochemical and Biophysical Research Communications. 2004 Mar;315(4):950-958.
Link to:	http://dx.doi.org/10.1016/j.bbrc.2004.01.151
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000220105600026
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84983723471
Appears in Collections:	[Yuh-Shan Jou(1996-2005)] Periodical Articles [Chiung-Tong Chen] Periodical Articles [Shiou-Hwei Yeh(2001-2005)] Periodical Articles

Files in This Item:

File	Description	Size	Format
000220105600026.pdf		873Kb	Adobe PDF	1195	View/Open

Loading...