國家衛生研究院 NHRI:Item 3990099045/2081

NHRI > National Institute of Infectious Diseases and Vaccinology > Ih-Jen Su(2002-2015) > Periodical Articles > Item 3990099045/2081

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2081

Title:	Differential endoplasmic reticulum stress signaling pathways mediated by iNOS
Authors:	Hsieh, YH;Su, IJ;Lei, HY;Lai, MD;Chang, WW;Huang, WY
Contributors:	Division of Clinical Research
Abstract:	Accumulated misfolded proteins in endoplasmic reticulum (ER) activate ER stress signaling pathways. Here we identified the ER factors that generate ROS molecules. After mouse NIH3T3 cells were treated with either tunicamycin or thapsigargin, oxidative stress was induced. We found inducible nitric oxide synthase (iNOS) was involved in the generation of ROS induced by ER stress. When thapsigargin-treated cells were pre-treated with iNOS inhibitors 1400W or L-canavanine, their ER stress-induced oxidative stress was almost totally abolished. This effect was not seen in the cells treated with tunicamycin. Therefore, iNOS appears to mediate the ER stress subpathway caused by Ca2+ efflux. To the contrary, after we treated the cells with the 26S proteasome inhibitors lactacystin or MG-132, the UPR-induced oxidative stress dramatically increased, indicating that clearing misfolded proteins from the ER lumen reduced the oxidative stress. Therefore, the oxidative stress induced by ER stress signaling is mediated through both iNOS-dependent and -independent subpathways. (c) 2007 Elsevier Inc. All rights reserved.
Keywords:	Biochemistry & Molecular Biology;Biophysics
Date:	2007-08-03
Relation:	Biochemical and Biophysical Research Communications. 2007 Aug;359(3):643-648.
Link to:	http://dx.doi.org/10.1016/j.bbrc.2007.05.154
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000247582500040
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34250169442
Appears in Collections:	[Ih-Jen Su(2002-2015)] Periodical Articles

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