國家衛生研究院 NHRI:Item 3990099045/2058

NHRI > National Institute of Infectious Diseases and Vaccinology > Hsiu-Jung Lo > Periodical Articles > Item 3990099045/2058

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2058

Title:	The roles of mutations in gyrA, parC, and ompK35 in fluoroquinolone resistance in Klebsiella pneumoniae
Authors:	Chen, FJ;Lauderdale, TL;Ho, M;Lo, HJ
Contributors:	Division of Clinical Research
Abstract:	In a survey of 541 Klebsiella pneumoniae isolates from 44 hospitals in Taiwan, three distinct populations were identified by the disk diffusion method according to the disribution of zone diameters of ciprofloxacin. Isolates with resistant, reduced-susceptible, and susceptible to fluoroquinolone were defined as CIP zone diameters of less than or equal to15 mm, 16-26 mm, and greater than or equal to27 mm, respectively. Thus, in addition to 38 (7%) resistant isolates, there were 30 (5.5%) reduced-susceptible isolates and 473 (87.5%) susceptible isolates. A total of 34 isolates consisting of nine resistant, 13 reduced-susceptible, and 12 susceptible isolates were assessed for point mutations in gyrA and parC and the outer membrane profiles. The susceptibility to fluoroquinolone of 13 reduced-susceptible isolates was not altered in the presence of carbonyl cyanide m-chlorophenylhydrazone, an efflux inhibitor, showing that efflux is not a major contributor to reduced susceptibility. In addition to single mutation in gyrA, OmpK35 porin loss can also be the first step for developing fluoroquinolone resistance. No strain possesses a parC mutation without the simultaneous presence of a gyrA mutation, suggesting that mutations in parC play a complementary role for higher-level of fluoroquinolone resistance and fluoroquinolone resistance is a multistep process.
Keywords:	Infectious Diseases;Microbiology;Pharmacology & Pharmacy
Date:	2003-08-01
Relation:	Microbial Drug Resistance. 2003 Aug 1;9(3):265-271.
Link to:	http://dx.doi.org/10.1089/107662903322286472
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1076-6294&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000185014900005
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0042355374
Appears in Collections:	[Hsiu-Jung Lo] Periodical Articles [Monto Ho(1997-2002)] Periodical Articles [Tsai-Ling Yang Lauderdale] Periodical Articles [Feng-Jui Chen] Periodical Articles

Files in This Item:

File	Description	Size	Format
000185014900005.pdf		1058Kb	Adobe PDF	865	View/Open

Loading...