國家衛生研究院 NHRI:Item 3990099045/1963
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 917001      在线人数 : 1518
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/1963


    题名: Novel SHV-derived-extended-spectrum beta-lactamase, SHV-57, that confers resistance to ceftazidime but not cefazolin
    作者: Ma, L;Alba, J;Chang, FY;Ishiguro, M;Yamaguchi, K;Siu, LK;Ishii, Y
    贡献者: Division of Clinical Research
    摘要: A new SHV-derived extended-spectrum beta-lactamase, SHV-57, that confers high-level resistance to ceftazidime but not cefotaxime or cefazolin was identified from a national surveillance study conducted in Taiwan in 1998. An Escherichia coli isolate resistant to ampicillin, cephalothin, and ceftazidime but sensitive to cefoxitin, ceftriaxone, cefotaxime, imipenem, and a narrow-spectrum cephem (cefazolin) was isolated from the urine of a patient treated with beta-lactam antibiotics. Resistance to beta-lactams was conjugatively transferred with a plasmid of about 50 kbp. The pI of this enzyme was 8.3. The sequence of the gene was determined, and the open reading frame of the gene was found to consist of 861 bases (GenBank accession number AY223863). Kinetic parameters showed that SHV-57 had a poor affinity to cefazolin. The K-m value toward cefazolin (5.57 x 10(3) muM) was extremely high in. comparison to those toward ceftazidime (30.9 muM) and penicillin G (67 muM), indicating its low affinity to cefazolin. Although the K-m value of the beta-lactamase inhibitor was too high for the study of catalytic activity (k(cat), indicating the low k(cat) of SHV-57, the SHV-57 carrier was highly susceptible to a beta-lactam-p-lactamase inhibitor combination. Comparison of the three-dimensional molecular model of SHV-57 with that of the SHV-1 beta-lactamase suggests that the substitution of arginine for leucine-169 in the Omega loop is important for the substrate specificity.
    关键词: Microbiology;Pharmacology & Pharmacy
    日期: 2005-02
    關聯: Antimicrobial Agents and Chemotherapy. 2005 Feb;49(2):600-605.
    Link to: http://dx.doi.org/10.1128/AAC.49.2.600-605.2005
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000226709900019
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=12944291456
    显示于类别:[蕭樑基] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    000226709900019.pdf302KbAdobe PDF362检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈