國家衛生研究院 NHRI:Item 3990099045/1933

NHRI > National Institute of Infectious Diseases and Vaccinology > Kun-Yen Huang(1999-2004) > Periodical Articles > Item 3990099045/1933

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Title:	In vitro and in vivo activities of newer fluoroquinolones against Vibrio vulnificus
Authors:	Tang, HJ;Chang, MC;Ko, WC;Huang, KY;Lee, CL;Chuang, YC
Contributors:	Division of Clinical Research
Abstract:	The MICs of six fluoroquinolones as well as minocycline and cefotaxime for 46 clinical isolates of Vibrio vulnificus were determined by the agar dilution method. All the drugs tested had good activities against all isolates, with the MICs at which 90% of the isolates tested were inhibited (MIC(90)s) by five of the fluoroquinolones ranging between 0.03 and 0.06 mug/ml. The MIC90 of lomefloxacin, on the other hand, was 0.12 mug/ml. Time-kill studies were conducted with these agents and a clinical strain of V. vulniflcus, W5823. When approximately 5 x 10(5) CFU of V. vulnificus/ml was incubated with any one of the above-mentioned six fluoroquinolones at concentrations of two times the MIC, there was an inhibitory effect on V. vulnificus that persisted for more than 48 h with no noted regrowth. The efficacies of the fluoroquinolones were further evaluated in vivo in the mouse model of experimental V. vulnificus infection and compared to the efficacy of a combination therapy using cefotaxime plus minocycline. With an inoculum of 1.5 x 10(7) CFU, 28 (87.5%) of 32 mice in the cefotaxime-minocycline-treated group survived and 29 (91%) of the 32 mice in the moxifloxacintreated group survived while none of the 32 mice in the control group did. With an inoculum of 3.5 x 10(7) CFU, the difference in survival rates among groups of 15 mice treated with levofloxacin (13 of 15), moxifloxacin (10 of 15), gatifloxacin (10 of 15), sparfloxacin (11 of 15), ciprofloxacin (12 of 15), or lomefloxacin (10 of 15) was not statistically significant while none of the 15 mice treated with saline survived. We concluded that the newer fluoroquinolones as single agents are as effective as the cefotaxime-minocycline combination in inhibiting V. vulnificus both in vitro and in vivo.
Keywords:	Microbiology;Pharmacology & Pharmacy
Date:	2002-11
Relation:	Antimicrobial Agents and Chemotherapy. 2002 Nov;46(11):3580-3584.
Link to:	http://dx.doi.org/10.1128/AAC.46.11.3580-3584.2002
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000178712800036
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036839698
Appears in Collections:	[Kun-Yen Huang(1999-2004)] Periodical Articles

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