國家衛生研究院 NHRI:Item 3990099045/1909

NHRI > National Institute of Infectious Diseases and Vaccinology > Monto Ho(1997-2002) > Periodical Articles > Item 3990099045/1909

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1909

Title:	Outer membrane protein change combined with co-existing TEM-1 and SHV-1 beta-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae
Authors:	Wu, TL;Siu, LK;Su, LH;Lauderdale, TL;Lin, FM;Leu, HS;Lin, TY;Ho, M
Contributors:	Division of Clinical Research
Abstract:	Nine isolates of Klebsiella pneumoniae, obtained from one colonized and eight bacteraemic patients on a paediatric ward, were shown to be identical by PFGE, indicating an outbreak. Screening for extended-spectrum beta -lactamase (ESBL) production using the double-disc synergy test, Etest for ESBLs and agar diffusion tests indicated ESBL production. The isolates showed reduced susceptibility to cefotaxime but not to other third-generation cephalosporins. Molecular studies revealed production of TEM-1 and SHV-1 but no ESBLs were identified. Deficiency in expression of an outer membrane protein (OmpK35) was also observed. These observations led us to postulate that the extremely low level of OmpK35 expression and the co-existence of TEM-1 and SHV-1 resulted in an increased MIC of cefotaxime and the false designation of the isolates as ESBL producers. All the infected infants were treated with either third-generation cephalosporins alone or multiple antibiotics including a third-generation cephalosporin, and recovered and were discharged without sequelae.
Keywords:	Infectious Diseases;Microbiology;Pharmacology & Pharmacy
Date:	2001-06
Relation:	Journal of Antimicrobial Chemotherapy. 2001 Jun;47(6):755-761.
Link to:	http://dx.doi.org/10.1093/jac/47.6.755
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-7453&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000169302300003
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034993931
Appears in Collections:	[Monto Ho(1997-2002)] Periodical Articles [Tsai-Ling Yang Lauderdale] Periodical Articles [Leung-Kei Siu] Periodical Articles

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