國家衛生研究院 NHRI:Item 3990099045/16260

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Title:	Hydroxyl functionalized hexagonal boron nitride quantum dots as nanozyme for pesticides sensing through dual colorimetric and fluorometric platform: A combined experimental and theoretical study
Authors:	Hazarika, C;Neog, G;Roy, E;Gogoi, D;Das, MR;Sarmah, K;Guha, AK;Konwar, R;Matsagar, BM;Wu, KCW
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	The Hydroxyl-functionalized hexagonal boron nitride quantum dots (h-BN QDs) were synthesized using a hydrothermal technique in a water medium, exhibiting stability in water for over 90 days. The present study demonstrates two simple fluorescence and colorimetric techniques for sensing organophosphorus (OP) and organochloride (OC) pesticides (atrazine, simazine and chlorpyrifos) in an aqueous medium using hydroxylfunctionalized h-BN QDs. The sensing capability of h-BN QDs for these pesticides was determined to be in the range of 5-10 mu M using both techniques. The hydroxyl groups on the surface of the QDs enhanced dispersibility and enabled peroxidase-like catalytic activity in acidic conditions. The radical generation using hydroxylfunctionalized h-BN QDs in the presence of H2O2 upon 2,2 '-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) oxidation plays a significant role in the peroxidase reaction. The hydrogen bonding and it-it interactions between the pesticide molecules and ABTS can enhance the pesticide sensing performance. The limit of detection (LOD) using ABTS for atrazine, simazine, and chlorpyrifos was 6.11, 6.56, and 2.47 mu M, respectively. Fluorescence sensing, based on a turn ON/OFF mechanism, demonstrated high specificity and sensitivity with LODs of 5.59, 6.45, and 8.08 mu M for the same pesticides. The DFT studies revealed non-covalent bonding, such as hydrogen bonding, it-it interactions between the pesticides and the h-BN QDs, indicating binding affinities and stabilization energies of the complexes which can detect pesticides effectively. Atrazine exhibited higher binding affinity towards h-BN QDs, followed by simazine and chlorpyrifos. The h-BN QDs provide a new insight into bonding with pesticides, enhancing the peroxidase mimetic and fluorescence activity.
Date:	2024-11-15
Relation:	Chemical Engineering Journal. 2024 Nov 15;500:Article number 156365.
Link to:	http://dx.doi.org/10.1016/j.cej.2024.156365
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1385-8947&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001350405000001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85207934446
Appears in Collections:	[Chia-Wen Wu] Periodical Articles

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