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Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/16098
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| Title: | Collection of different types of cells from frozen umbilical cord stored by the innovative cryopreservation technology |
| Authors: | Yang, Y;Shih, Y;Ko, Y;Lin, C;Wei, H;Chiu, J |
| Contributors: | Institute of Cellular and Systems Medicine |
| Abstract: | Background & Aim: Umbilical cord (UC) is recognized as an advantageous cell source with broad therapeutic potential in clinical trials. In addition to the most commonly used mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs), several cell types can be isolated from UC. Recently, we have developed the advanced UCT III Plus cryopreservation technology (UCT III +, HealthBanks Biomedical) which aims to preserve therapeutic properties of MSCs, and also improve the cryopreservation efficiency of vascular lineage cells including endothelial cells (ECs), smooth muscle cells (SMCs) from UC tissues. In this study, the isolation of vascular cells from UCT III+ cryopreserved UC tissues have been characterized and analyzed. Furthermore, endothelial progenitor cells (EPCs) could be isolated from cryopreserved UC blood (UCB). The effects of UC cryopreservation on the viability and activities of these vascular lineage cells remain unclear. Methods, Results & Conclusion: Fresh human UC tissues and UCB were cryopreserved by optimized UCT III+ and CB techniques in HealthBanks Biomedical Co., Ltd. Vascular ECs, SMCs were isolated by explantation and enzymatic digestion methods. EPCs were isolated through density gradient centrifugation and expansion culture system from fresh and frozen UCB. The cells from pre-and post- cryopreserved UC tissues and their serial passages were compared. Cell morphology, viability, proliferation, specific protein expression and functions were evaluated. Thawed UCB contained CD34+CD133+ EPCs and exhibited active mitochondria membrane potential after being culture for 14 days. ECs can be successfully isolated from fresh and frozen UC tissues. The initial ECs population from cryopreserved UC tissues had a lower growth rate due to low cell yield. SMCs were outgrowth from thawed UC tissue arteries within 14 days. These SMCs highly expressed SMCspecific markers smooth muscle-22aand smooth muscle-a-actin. Moreover, the expanded thawed SMCs showed a high proliferate rate and exhibited excellent gel contraction capacities compared to the SMCs from fresh UC tissues. The results indicate that CD34+CD133+ EPCs from cryopreserved CB technique and vascular cells from UCT III Plus technique may retain their normal cellular viability and activities, suggesting that the UC cryopreservation platform may be beneficial for further regenerative medicine and vascular cell therapy applications. |
| Date: | 2024-06 |
| Relation: | Cytotherapy. 2024 Jun;26(6, Suppl.):S113. |
| Link to: | https://doi.org/10.1016/j.jcyt.2024.03.213 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1465-3249&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:001282333600260 |
| Appears in Collections: | [裘正健] 會議論文/會議摘要
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| ISI001282333600260.pdf | | 365Kb | Adobe PDF | 22 | View/Open |
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