國家衛生研究院 NHRI:Item 3990099045/15900
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    题名: Kaempferol-enhanced migration and differentiation of C2C12 myoblasts via ITG1B/FAK/Paxillin and IGF1R/AKT/mTOR signaling pathways
    作者: Hour, TC;Nhi, NTL;Lai, IJ;Chuu, CP;Lin, PC;Chang, HW;Su, YF;Chen, CH;Chen, YK
    贡献者: Institute of Cellular and Systems Medicine
    摘要: ScopeKaempferol (KMP), a bioactive flavonoid compound found in fruits and vegetables, contributes to human health in many ways but little is known about its relationship with muscle mass. The effect of KMP on C2C12 myoblast differentiation and the mechanisms that might underlie that effect are studied.Methods and resultsThis study finds that KMP (1, 10 mu M) increases the migration and differentiation of C2C12 myoblasts in vitro. Studying the possible mechanism underlying its effect on migration, the study finds that KMP activates Integrin Subunit Beta 1 (ITGB1) in C2C12 myoblasts, increasing p-FAK (Tyr398) and its downstream cell division cycle 42 (CDC42), a protein previously associated with cell migration. Regarding differentiation, KMP upregulates the expression of myosin heavy chain (MHC) and activates IGF1/AKT/mTOR/P70S6K. Interestingly, pretreatment with an AKT inhibitor (LY294002) and siRNA knockdown of IGF1R leads to a decrease in cell differentiation, suggesting that IGF1/AKT activation is required for KMP to induce C2C12 myoblast differentiation.ConclusionTogether, the findings suggest that KMP enhances the migration and differentiation of C2C12 myoblasts through the ITG1B/FAK/paxillin and IGF1R/AKT/mTOR pathways. Thus, KMP supplementation might potentially be used to prevent or delay age-related loss of muscle mass and help maintain muscle health. Schematic diagram of KMP regulation of C2C12 myoblast migration and myogenic differentiation. AKT, protein kinase B; CDC42, cell division cycle 42; ECM, cell-extracellular matrix; FAK, focal adhesion kinase; IGF1R, insulin-like growth factor 1 receptor; ITGB1, integrin subunit beta 1; MHC, myosin heavy chain; mTOR, mammalian target of rapamycin; P70S6K, ribosomal protein S6 kinase beta-1/p70S6 kinase. image
    日期: 2024-06-11
    關聯: Molecular Nutrition and Food Research. 2024 Jun 11;Article in Press.
    Link to: http://dx.doi.org/10.1002/mnfr.202300685
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1613-4125&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:001242907600001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85195473250
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