國家衛生研究院 NHRI:Item 3990099045/15881

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Title:	Exploring multi-faceted bioactivity of Abeta42 oligomers on diverse bio-substrates: Implications for Alzheimer's disease and cerebral amyloid angiopathy
Other Titles:	Exploring multi-faceted bioactivity of Aβ42 oligomers on diverse bio-substrates: Implications for Alzheimer's disease and cerebral amyloid angiopathy
Authors:	Huang, CJ;Ho, SY;Wu, SH;Wang, YL;Dong, GC
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	Background: Charge and hydrophobicity are key factors for the construction of bioactive proteins on bio-substrates. Amyloid-beta (Aβ) is a biomarker of Alzheimer's disease and cerebral amyloid angiopathy, and this study explores its multi-faceted bioactivity guided by bio-substrates. Methods: Initially, the dynamic mode was used to confirm the homemade rhAβ42, and then bio-substrates and metal ions were screened to construct rhAβ42 oligomers. In the static mode, hydrophobicity and morphology were analyzed through contact angle and microscopy, and WST-1 was used to investigate the bioactivity of monomers and oligomers constructed on bio-substrates. Significant findings: The results show that the homemade rhAβ42 and rhAβ-Cu oligomers are not only recognized by specific antibodies, but also Protein G and Type B gelatin combined with ferric were constructed as rhAβ-Fe oligomers in this dynamic model. Static pattern analysis indicates that Type B gelatin had the relatively highest hydrophobicity and directly affects rhAβ42 binding. Morphologically, Protein G and Type B gelatin are used as bio-substrates to construct two-dimensional leaf vein-like and three-dimensional columnar structures, and there is a clear difference between 10 % promotion and 70 % mortality at different bioactive profiles. We hope to contribute to the cerebral amyloid angiopathy-related research and in vitro pathogenic microenvironmental models in the future.
Date:	2024-10
Relation:	Journal of the Taiwan Institute of Chemical Engineers. 2024 Oct;163:Article number 105570.
Link to:	http://dx.doi.org/10.1016/j.jtice.2024.105570
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1876-1070&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001317003800001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85194379414
Appears in Collections:	[Guo-Chung Dong] Periodical Articles

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