國家衛生研究院 NHRI:Item 3990099045/15688

NHRI > National Institute of Infectious Diseases and Vaccinology > Chia-Yu Chi > Periodical Articles > Item 3990099045/15688

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15688

Title:	Conserved molecular chaperone PrsA stimulates protective immunity against group a Streptococcus
Authors:	Lai, CY;Xie, JX;Lai, MC;Wu, ZY;Lin, JS;Huang, YT;Chi, CY;Chiang-Ni, C;Walker, MJ;Chang, YC
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	Group A Streptococcus (GAS) is a significant human pathogen that poses a global health concern. However, the development of a GAS vaccine has been challenging due to the multitude of diverse M-types and the risk of triggering cross-reactive immune responses. Our previous research has identified a critical role of PrsA1 and PrsA2, surface post-translational molecular chaperone proteins, in maintaining GAS proteome homeostasis and virulence traits. In this study, we aimed to further explore the potential of PrsA1 and PrsA2 as vaccine candidates for preventing GAS infection. We found that PrsA1 and PrsA2 are highly conserved among GAS isolates, demonstrating minimal amino acid variation. Antibodies specifically targeting PrsA1/A2 showed no cross-reactivity with human heart proteins and effectively enhanced neutrophil opsonophagocytic killing of various GAS serotypes. Additionally, passive transfer of PrsA1/A2-specific antibodies conferred protective immunity in infected mice. Compared to alum, immunization with CFA-adjuvanted PrsA1/A2 induced higher levels of Th1-associated IgG isotypes and complement activation and provided approximately 70% protection against invasive GAS challenge. These findings highlight the potential of PrsA1 and PrsA2 as universal vaccine candidates for the development of an effective GAS vaccine.
Date:	2024-02-26
Relation:	NPJ Vaccines. 2024 Feb 26;9:Article number 46.
Link to:	http://dx.doi.org/10.1038/s41541-024-00839-7
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2059-0105&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001177366300001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85185928985
Appears in Collections:	[Chia-Yu Chi] Periodical Articles

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