國家衛生研究院 NHRI:Item 3990099045/15651

NHRI > Institute of Biotechnology and Pharmaceutical Research > Chun-Wei Tung > Periodical Articles > Item 3990099045/15651

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15651

Title:	Fipronil disturbs the antigen-specific immune responses and GABAergic gene expression in the ovalbumin-immunized BALB/c mice
Authors:	Kuo, JF;Cheng, YH;Tung, CW;Wang, CC
Contributors:	Institute of Biotechnology and Pharmaceutical Research
Abstract:	Background: Fipronil (FPN) is a broad-spectrum pesticide and commonly known as low toxicity to vertebrates. However, increasing evidence suggests that exposure to FPN might induce unexpected adverse effects in the liver, reproductive, and nervous systems. Until now, the influence of FPN on immune responses, especially T-cell responses has not been well examined. Our study is designed to investigate the immunotoxicity of FPN in ovalbumin (OVA)-sensitized mice. The mice were administered with FPN by oral gavage and immunized with OVA. Primary splenocytes were prepared to examine the viability and functionality of antigen-specific T cells ex vivo. The expression of T cell cytokines, upstream transcription factors, and GABAergic signaling genes was detected by qPCR. Results: Intragastric administration of FPN (1–10 mg/kg) for 11 doses did not show any significant clinical symptoms. The viability of antigen-stimulated splenocytes, the production of IL-2, IL-4, and IFN-γ by OVA-specific T cells, and the serum levels of OVA-specific IgG1 and IgG2a were significantly increased in FPN-treated groups. The expression of the GABAergic signaling genes was notably altered by FPN. The GAD67 gene was significantly decreased, while the GABAR β2 and GABAR δ were increased. Conclusion: FPN disturbed antigen-specific immune responses by affecting GABAergic genes in vivo. We propose that the immunotoxic effects of FPN may enhance antigen-specific immunity by dysregulation of the negative regulation of GABAergic signaling on T cell immunity.
Date:	2024-01-22
Relation:	BMC Veterinary Research. 2024 Jan 22;20:Article number 30.
Link to:	http://dx.doi.org/10.1186/s12917-024-03878-3
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1746-6148&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001153475700001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85182812689
Appears in Collections:	[Chun-Wei Tung] Periodical Articles

Files in This Item:

File	Description	Size	Format
SCP85182812689.pdf		1666Kb	Adobe PDF	74	View/Open

Loading...