國家衛生研究院 NHRI:Item 3990099045/15251
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15251


    Title: Targeting conserved oathways in 3D spheroid Formation of diverse cell types for translational application: Enhanced functional and antioxidant capacity
    Authors: Chang, CC;Jiang, SS;Tsai, FY;Hsu, PJ;Hsieh, CC;Wang, LT;Yen, ML;Yen, BL
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: Three-dimensional (3D) in vitro spheroid/organoid culture increasingly appears to better mimic physiological states than standard 2D systems. The biological consequence of 3D spheroids, however, differs for different cell types: for pluripotent embryonic stem cells (ESCs), differentiation and loss of stemness occur, while the converse is true for somatic and cancer cells. Despite such diverse consequences, there are likely conserved mechanisms governing 3D spheroid formation across cell types that are unknown but could be efficiently targeted for translational application. To elucidate such processes, we performed transcriptome analysis with functional validation on 2D- and 3D-cultured mouse ESCs, mesenchymal stromal/stem cells (MSCs), and cancer cells. At both the transcriptomic and functional levels, 3D spheroid formation resulted in commitment towards known cell-specific functional outcomes. Surprisingly in all cell types, downregulation of the cholesterol synthesis pathway was found during 3D spheroid formation, with modulation concomitantly affecting 3D spheroid formation and cell-specific consequences; similar results were seen with human cell types. Furthermore, improved antioxidant capacity after 3D spheroid formation across cell types was further enhanced with modulation of the pathway. These findings demonstrate the profound cell-specific consequences and the translational value of understanding conserved mechanisms across diverse cell types after 3D spheroid formation.
    Date: 2023-08-11
    Relation: Cells. 2023 Aug 11;12(16):Article number 2050.
    Link to: http://dx.doi.org/10.3390/cells12162050
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2073-4409&DestApp=IC2JCR
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85168729076
    Appears in Collections:[Betty Lin-Ju Yen] Periodical Articles

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