國家衛生研究院 NHRI:Item 3990099045/15218
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15218


    Title: A microfluidic countercurrent reactor for accelerating enzymatic reactions
    Authors: Juang, DS;Huo, YR;Tseng, HK;Chen, CC;Hsu, CH;Lin, CC
    Contributors: Institute of Biomedical Engineering and Nanomedicine
    Abstract: Enzymes are important catalysts in biochemical reactions with superior regio, stereo, and substrate selectivity. However, enzymatic reaction systems have drawbacks including product inhibition, difficulty recycling, and poor stability. Importantly, the rate of an enzyme catalyzed reaction diminishes rapidly due to product inhibition and substrate depletion, making it difficult for many enzymes to catalyze a reaction to completion. The outcome is a mixture of unreacted substrates being present in the final reaction, necessitating additional separation steps that increase costs. This study presents a microfluidic reactor for accelerating enzyme catalyzed reactions using a countercurrent design that continuously removes products and adds fresh substrate into the reaction, allowing enzymes to operate under better reaction conditions. It demonstrates that countercurrent flow accelerates enzymatic reactions in our system up to 36 % for horseradish peroxidase and 21 % for & beta;-glucosidase compared to cocurrent flow, and the resulting reaction solution contains highly pure product with minimal substrate contamination.
    Date: 2023-08-08
    Relation: ChemCatChem. 2023 Aug 08;15(17):Article number e202300304.
    Link to: http://dx.doi.org/10.1002/cctc.202300304
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1867-3880&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:001040524900001
    Cited Times(Scopus): 2-s2.0-85166254447
    https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85166254447
    Appears in Collections:[Chia-Hsien Hsu] Periodical Articles

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