國家衛生研究院 NHRI:Item 3990099045/1488

NHRI > Institute of Biomedical Engineering and Nanomedicine > Jeng-Jiann Chiu > Periodical Articles > Item 3990099045/1488

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1488

Title:	Neutrophils, lymphocytes, and monocytes exhibit diverse behaviors in transendothelial and subendothelial migrations under coculture with smooth muscle cells in disturbed flow
Authors:	Chen, CN;Chang, SF;Lee, PL;Chang, K;Chen, LJ;Usami, S;Chien, S;Chiu, JJ
Contributors:	Division of Medical Engineering Research
Abstract:	Atherosclerosis develops at regions of the arterial tree exposed to disturbed flow. The early stage of atherogenesis involves the adhesion of leukocytes (white blood cells [WBCs]) to and their transmigration across endothelial cells (ECs), which are located in close proximity to smooth muscle cells (SMCs). We investigated the effects of EC/SMC coculture and disturbed flow on the adhesion and transmigration of 3 types of WBCs (neutrophils, peripheral blood lymphocytes [PBLs], and monocytes) using our vertical-step flow (VSF) chamber, in which ECs were cocultured with SMCs in collagen gels. Such coculture significantly increased the adhesion and transmigration of neutrophils, PBLs, and monocytes under VSF, particularly in the reattachment area, where the rolling velocity of WBCs and their transmigration time were decreased, as compared with the other areas. Neutrophils, PBLs, and monocytes showed different subendothelial migration patterns under VSF. Their movements were more random and shorter in distance in the reattachment area. Coculture of ECs and SMCs induced their expressions of adhesion molecules and chemokines, which contributed to the increased WBC adhesion and transmigration. Our findings provide insights into the mechanisms of WBC interaction with the vessel wall (composed of ECs and SMCs) under the complex flow environments found in regions of prevalence for atherogenesis.
Keywords:	Hematology
Date:	2006-03-01
Relation:	Blood. 2006 Mar;107(5):1933-1942.
Link to:	http://dx.doi.org/10.1182/blood-2005-08-3137
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-4971&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000235632700035
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33344466119
Appears in Collections:	[Jeng-Jiann Chiu] Periodical Articles

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