國家衛生研究院 NHRI:Item 3990099045/14695

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14695

Title:	Revisiting the cerebral hemodynamics of awake, freely moving rats with repeated ketamine self-administration using a miniature photoacoustic imaging system
Authors:	Wang, Y;Tsai, CH;Chu, TS;Hung, YT;Lee, MY;Chen, HH;Chen, LT;Ger, TR;Wang, YH;Chiang, NJ;Liao, LD
Contributors:	Institute of Biomedical Engineering and Nanomedicine;Center for Neuropsychiatric Research;National Institute of Cancer Research
Abstract:	SIGNIFICANCE: Revealing the dynamic associations between brain functions and behaviors is a significant challenge in neurotechnology, especially for awake subjects. Imaging cerebral hemodynamics in awake animal models is important because the collected data more realistically reflect human disease states. AIM: We previously reported a miniature head-mounted scanning photoacoustic imaging (hmPAI) system. In the present study, we utilized this system to investigate the effects of ketamine on the cerebral hemodynamics of normal rats and rats subjected to prolonged ketamine self-administration. APPROACH: The cortical superior sagittal sinus (SSS) was continuously monitored. The full-width at half-maximum (FWHM) of the photoacoustic (PA) A-line signal was used as an indicator of the SSS diameter, and the number of pixels in PA B-scan images was used to investigate changes in the cerebral blood volume (CBV). RESULTS: We observed a significantly higher FWHM (blood vessel diameter) and CBV in normal rats injected with ketamine than in normal rats injected with saline. For rats subjected to prolonged ketamine self-administration, no significant changes in either the blood vessel diameter or CBV were observed. CONCLUSIONS: The lack of significant change in prolonged ketamine-exposed rats was potentially due to an increased ketamine tolerance. Our device can reliably detect changes in the dilation of cortical blood vessels and the CBV. This study validates the utility of the developed hmPAI system in an awake, freely moving rat model for behavioral, cognitive, and preclinical cerebral disease studies.
Date:	2022-11-01
Relation:	Neurophotonics. 2022 Nov 01;9(4):Article number 045003.
Link to:	http://dx.doi.org/10.1117/1.NPh.9.4.045003
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2329-423X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000926179900014
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85146149536
Appears in Collections:	[Lun-De Liao] Periodical Articles [Hwei-Hisen Chen] Periodical Articles [Nai-Jung Chiang] Periodical Articles [Li-Tzong Chen] Periodical Articles

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