國家衛生研究院 NHRI:Item 3990099045/1451

NHRI > Institute of Biomedical Engineering and Nanomedicine > Kurt Ming-Chao Lin > Periodical Articles > Item 3990099045/1451

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Title:	Overexpression of PHGPx and HSP60/10 protects against ischemia/reoxygenation injury
Authors:	Hollander, JM;Lin, KM;Scott, BT;Dillmann, WH
Contributors:	Division of Medical Engineering Research
Abstract:	Reactive oxygen species arising from ischemia/reperfusion (I/R) cause damage to cardiac tissue. We examined the effects of mitochondrial phospholipid hydroperoxide glutathione peroxidase (mPHGPx) and cytosolic PHGPx (cPHGPx) overexpression on protection against simulated I/R in neonatal rat cardiac myocytes (NCM). Additionally, a protective combinatorial effect with heat shock proteins 60 and 10 (HSP60/10) was investigated. NCM were infected with adenoviral vectors expressing mPHGPx, cPHGPx, HSP60/10, or an empty control (Adv-) and submitted to 8 h of ischemia followed by 16 h of reoxygenation. mPHGPx infection led to a 40% decrease in malondialdehyde and 4-hydroxy-2(E)-nonenal following I/R (p < .05). Creatine kinase and lactate dehydrogenase release were decreased in both mPHGPx-infected and HSP60/10-infected cells (p < .05). The combination of mPHGPx and HSP60/10 overexpression led to further protection (p < .01). DNA laddering and histone-associated DNA fragments were decreased in PHGPx- and HSP60/10-infected cells (p < .01). Cytochrome c release from mitochondria was decreased in mPHGPx-infected cells. Furthermore, mPHGPx overexpression preserved electron transport chain complex IV function following simulated I/R (p < .05). These results indicate that overexpression of PHGPx provides protection against damage resulting from simulated I/R injury, particularly in the mitochondria, and that the combination of mPHGPx and HSP60/10 imparts an added protective effect. (C) 2003 Elsevier Inc.
Keywords:	Biochemistry & Molecular Biology;Endocrinology & Metabolism
Date:	2003-10-01
Relation:	Free Radical Biology and Medicine. 2003 Oct;35(7):742-751.
Link to:	http://dx.doi.org/10.1016/S0891-5849(03)00400-3
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0891-5849&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000185520900005
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0142258709
Appears in Collections:	[Kurt Ming-Chao Lin] Periodical Articles

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