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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14272


    Title: Furin and TMPRSS2 resistant spike induces robust humoral and cellular immunity against SARS-CoV-2 lethal infection
    Authors: Lin, JJ;Tien, CF;Kuo, YP;Lin, EJ;Tsai, WH;Chen, MY;Tsai, PJ;Su, YW;Pathak, N;Yang, JM;Yu, CY;Chuang, ZS;Wu, HC;Tsai, WT;Dai, SS;Liao, HC;Chai, KM;Su, YS;Chuang, TH;Liu, SJ;Chen, HW;Dou, HY;Chen, FJ;Chen, CT;Liao, CL;Yu, GY
    Contributors: National Institute of Infectious Diseases and Vaccinology;Immunology Research Center;Institute of Biotechnology and Pharmaceutical Research
    Abstract: An effective COVID-19 vaccine against broad SARS-CoV-2 variants is still an unmet need. In the study, the vesicular stomatitis virus (VSV)-based vector was used to express the SARS-CoV-2 Spike protein to identify better vaccine designs. The replication-competent of the recombinant VSV-spike virus with C-terminal 19 amino acid truncation (S Delta 19 Rep) was generated. A single dose of S Delta 19 Rep intranasal vaccination is sufficient to induce protective immunity against SARS-CoV-2 infection in hamsters. All the clones isolated from the S Delta 19 Rep virus contained R682G mutation located at the Furin cleavage site. An additional S813Y mutation close to the TMPRSS2 cleavage site was identified in some clones. The enzymatic processing of S protein was blocked by these mutations. The vaccination of the R682G-S813Y virus produced a high antibody response against S protein and a robust S protein-specific CD8(+) T cell response. The vaccinated animals were protected from the lethal SARS-CoV-2 (delta variant) challenge. The S antigen with resistance to enzymatic processes by Furin and TMPRSS2 will provide better immunogenicity for vaccine design.
    Date: 2022-05-02
    Relation: Frontiers in Immunology. 2022 May 2;13:Article number 872047.
    Link to: http://dx.doi.org/10.3389/fimmu.2022.872047
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1664-3224&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000796928300001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85130223487
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