國家衛生研究院 NHRI:Item 3990099045/14188

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/14188

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/14188

Title:	A portable controllable compressive stress device to monitor human breast cancer cell protrusions at single-cell resolution
Authors:	Yeh, CF;Juang, DS;Chen, YW;Rodoplu, D;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine;National Institute of Cancer Research
Abstract:	In vitro devices offer more numerous methods than in vivo models to investigate how cells respond to pressure stress and quantify those responses. Several in vitro devices have been developed to study the cell response to compression force. However, they are unable to observe morphological changes of cells in real-time. There is also a concern about cell damage during the process of harvesting cells from 3D gels. Here we report a device employing transparent, thin gel layers to clamp cells between the interfaces and applied a controllable compression force by stacking multiple layers on the top. In this approach, cells can be monitored for alteration of cellular protrusions, whose diversity has been proven to promote cancer cell dissemination, with single-cell resolution under compression force. Furthermore, p-Rac-1 and rhodamine staining on the device directly to confirm the actin filaments of lamellipodia. The method was able to fulfill real-time live-cell observation at single-cell resolution and can be readily used for versatile cell analysis. MDA-MB-231 and MCF7 breast cancer cells were utilized to demonstrate the utility of the device, and the results showed that the stimuli of compression force induce MDA-MB-231 and MCF7 to form lamellipodia and bleb protrusions, respectively. We envision the device may be used as a tool to explore mechanisms of membrane protrusion transitions and to screen drug candidates for inhibiting cancer cell protrusion plasticity for cancer therapy.
Date:	2022-02
Relation:	Frontiers in Bioengineering and Biotechnology. 2022 Feb;10:Article number 852318.
Link to:	http://dx.doi.org/10.3389/fbioe.2022.852318
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2296-4185&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000769357000001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85126190842
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles [Ya-Wen Chen] Periodical Articles

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