國家衛生研究院 NHRI:Item 3990099045/1406
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1406


    Title: Characterization of cytokeratin 19-positive hepatocyte foci in the regenerating rat liver after 2-AAF/CCl4 injury
    Authors: Chiu, CC;Huang, GT;Chou, SH;Chien, CT;Chiou, LL;Chang, MH;Lee, HS;Chen, DS
    Contributors: Stem Cell Research Center
    Abstract: Partial hepatectomy or carbon tetrachloride (CCl4) injury, following treatment of rats with 2-acetylaminofluorene (2-AAF) to inhibit proliferation of hepatocytes, induces proliferation of oval cells and possibly their differentiation into nodular foci of hepatocytes when higher doses of 2-AAF are used. Unfortunately, immunohistochemistry in previous studies failed to show oval cell markers in these foci, and thereby to demonstrate the precursor-product relationship between oval cells and hepatocytes. Immunohistochemistry on livers of rats treated with high dose 2-AAF/CCl4 was used. We found 7.6% of the hepatocyte foci were positive for an oval cell marker cytokeratin 19 (CK-19). These foci were positive for alpha-fetoprotein, less positive for carbamoylphosphate synthetase 1, and more positive for laminin in the basement membrane lining. Rarely present transitional foci had weaker expression of CK-19 and discontinuous laminin. Focal hepatocyte differentiation of oval cells was characterized by cell hypertrophy, membranous CK-19, and positive hepatocyte nuclear factor 4 (HNF-4). HNF-4(+) small oval cells surrounding CK-19(+) foci were frequently seen, suggesting that a paracrine mechanism(s) may be responsible for the enlargement of CK-19(+) foci. In conclusions, oval cells appear to differentiate to CK-19(+) foci and then to CK-19(-) foci in the high dose 2-AAF/CCl4 model.
    Keywords: Cell Biology;Microscopy
    Date: 2007-09
    Relation: Histochemistry and Cell Biology. 2007 Sep;128(3):217-226.
    Link to: http://dx.doi.org/10.1007/s00418-007-0309-3
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0948-6143&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000249019200004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34548162822
    Appears in Collections:[Others] Periodical Articles

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