國家衛生研究院 NHRI:Item 3990099045/13868

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Title:	Conditioned media of adipose-derived stem cells suppresses sidestream cigarette smoke extract induced cell death and epithelial-mesenchymal transition in lung epithelial cells
Authors:	Chen, TY;Liu, CH;Chen, TH;Lin, KMC;Chen, MR;Liu, SW;Lin, P
Contributors:	Institute of Biomedical Engineering and Nanomedicine;National Institute of Environmental Health Sciences;Institute of Population Health Sciences
Abstract:	The role of the epithelial–mesenchymal transition (EMT) in lung epithelial cells is increasingly being recognized as a key stage in the development of COPD, fibrosis, and lung cancers, which are all highly associated with cigarette smoking and with exposure to second-hand smoke. Using the exposure of human lung cancer epithelial A549 cells and non-cancerous Beas-2B cells to sidestream cigarette smoke extract (CSE) as a model, we studied the protective effects of adipose-derived stem cell-conditioned medium (ADSC-CM) against CSE-induced cell death and EMT. CSE dose-dependently induced cell death, decreased epithelial markers, and increased the expression of mesenchymal markers. Upstream regulator analysis of differentially expressed genes after CSE exposure revealed similar pathways as those observed in typical EMT induced by TGF-β1. CSE-induced cell death was clearly attenuated by ADSC-CM but not by other control media, such as a pass-through fraction of ADSC-CM or A549-CM. ADSC-CM effectively inhibited CSE-induced EMT and was able to reverse the gradual loss of epithelial marker expression associated with TGF-β1 treatment. CSE or TGF-β1 enhanced the speed of A549 migration by 2-to 3-fold, and ADSC-CM was effective in blocking the cell migration induced by either agent. Future work will build on the results of this in vitro study by defining the molecular mechanisms through which ADSC-CM protects lung epithelial cells from EMT induced by toxicants in second-hand smoke.
Date:	2021-11-08
Relation:	International Journal of Molecular Sciences. 2021 Nov 8;22(21):Article number 12069.
Link to:	http://dx.doi.org/10.3390/ijms222112069
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1422-0067&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000719524400001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85118479308
Appears in Collections:	[Kurt Ming-Chao Lin] Periodical Articles [Pinpin Lin] Periodical Articles [Others] Periodical Articles

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