Loading...
|
Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/13680
|
| Title: | Remdesivir and cyclosporine synergistically inhibit the human coronaviruses OC43 and SARS-CoV-2 |
| Authors: | Hsu, HY;Yang, CW;Lee, YZ;Lin, YL;Chang, SY;Yang, RB;Liang, JJ;Chao, TL;Liao, CC;Kao, HC;Wu, SH;Chang, JY;Sytwu, HK;Chen, CT;Lee, SJ |
| Contributors: | Institute of Biotechnology and Pharmaceutical Research;National Institute of Infectious Diseases and Vaccinology |
| Abstract: | Remdesivir, a prodrug targeting RNA-dependent-RNA-polymerase, and cyclosporine, a calcineurin inhibitor, individually exerted inhibitory activity against human coronavirus OC43 (HCoV-OC43) in HCT-8 and MRC-5 cells at EC50 values of 96 ± 34 ∼ 85 ± 23 nM and 2,920 ± 364 ∼ 4,419 ± 490 nM, respectively. When combined, these two drugs synergistically inhibited HCoV-OC43 in both HCT-8 and MRC-5 cells assayed by immunofluorescence assay (IFA). Remdesivir and cyclosporine also separately reduced IL-6 production induced by HCoV-OC43 in human lung fibroblasts MRC-5 cells with EC50 values of 224 ± 53 nM and 1,292 ± 352 nM, respectively; and synergistically reduced it when combined. Similar trends were observed for SARS-CoV-2, which were 1) separately inhibited by remdesivir and cyclosporine with respective EC50 values of 3,962 ± 303 nM and 7,213 ± 143 nM by IFA, and 291 ± 91 nM and 6,767 ± 1,827 nM by a plaque-formation assay; and 2) synergistically inhibited by their combination, again by IFA and plaque-formation assay. Collectively, these results suggest that the combination of remdesivir and cyclosporine merits further study as a possible treatment for COVID-19 complexed with a cytokine storm. |
| Date: | 2021-08-13 |
| Relation: | Frontiers in Pharmacology. 2021 Aug 13;12:Article number 706901. |
| Link to: | http://dx.doi.org/10.3389/fphar.2021.706901 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1663-9812&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000693446800001 |
| Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85114176838 |
| Appears in Collections: | [李秀珠] 期刊論文
[陳炯東] 期刊論文
[張俊彥] 期刊論文
[司徒惠康] 期刊論文
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| SCP85114176838.pdf | | 3804Kb | Adobe PDF | 304 | View/Open |
|
All items in NHRI are protected by copyright, with all rights reserved.
|
