國家衛生研究院 NHRI:Item 3990099045/1346

NHRI > National Institute of Infectious Diseases and Vaccinology > Shih-Jen Liu > Periodical Articles > Item 3990099045/1346

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1346

Title:	Identification of synthetic vaccine candidates against SARS CoV infection
Authors:	Lien, SP;Shih, YP;Chen, HW;Tsai, JP;Leng, CH;Lin, MH;Lin, LH;Liu, HY;Chou, AH;Chang, YW;Chen, YMA;Chong, P;Liu, SJ
Contributors:	Vaccine Research and Development Center
Abstract:	Three peptides, D1 (amino acid residues 175-201), D2 (a.a. 434-467), and TM (a.a. 1128-1159), corresponding to the spike protein (S) of severe acute respiratory syndrome corona virus (SARS CoV) were synthesized and their immunological functions were investigated in three different animals models (mice, guinea pigs, and rabbits). The peptides mixture formulated either with Freund's adjuvant or synthetic adjuvant Montanide ISA-51/oligodeoxy nucleotide CpG (ISA/CpG) could elicit antisera in immunized animals which were capable of inhibiting SARS/HIV pseudovirus entry into HepG2 cells. The neutralizing epitopes were identified using peptides to block the neutralizing effect of guinea pig antisera. The major neutralizing epitope was located on the D2 peptide, and the amino acid residue was fine mapped to 434-453. In BALB/c mice T-cell proliferation assay revealed that only D2 peptide contained T-cell epitope, the sequence of which corresponded to amino acid residue 434-448. The ISA/CpG formulation generated anti-132 IgG titer comparable to those obtained from Freund's adjuvant formulation, but generated fewer antibodies against D1 or TM peptides. The highly immunogenic D2 peptide contains both neutralizing and Th cell epitopes. These results suggest that synthetic peptide D2 would be useful as a component of SARS vaccine candidates. (c) 2007 Elsevier Inc. All rights reserved.
Keywords:	Biochemistry & Molecular Biology;Biophysics
Date:	2007-07-06
Relation:	Biochemical and Biophysical Research Communications. 2007 Jul;358(3):716-721.
Link to:	http://dx.doi.org/10.1016/j.bbrc.2007.04.164
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000247124900008
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34248993523
Appears in Collections:	[Shih-Jen Liu] Periodical Articles [Chih-Hsiang Leng] Periodical Articles [Pele Choi-Sing Chong] Periodical Articles [Hsin-Wei Chen] Periodical Articles

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