國家衛生研究院 NHRI:Item 3990099045/1307
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    題名: HLA-A*0201 T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus nucleocapsid and spike proteins
    作者: Tsao, YP;Lin, JY;Jan, JT;Leng, CH;Chu, CC;Yang, YC;Chen, SL
    貢獻者: Vaccine Research and Development Center
    摘要: The immunogenicity of HLA-A*0201-restricted cytotoxic T lymphocyte (CTL) peptide in severe acute respiratory syndrome coronavirus (SARS-CoV) nuclear capsid (N) and spike (S) proteins was determined by testing the proteins' ability to elicit a specific cellular immune response after immunization of HILA-A2.1 transgenic mice and in vitro vaccination of HLA-A2.1 positive human peripheral blood mononuclearcytes (PBMCs). First, we screened SARS N and S amino acid sequences for allele-specific motif matching those in human HLA-A2.1 MHC-1 molecules. From HILA peptide binding predictions (http://thr.cit.nih.gov/i-nolbio/hia-bitid/),. ten each potential N- and S-specific HLA-A2. 1-binding peptides were synthesized. The high affinity HLA-A2.1 peptides were validated by T2-cell stabilization assays, with immunogenicity assays revealing peptides N223-231, N227-235, and N317-325 to be the first identified H LA, previous reports identified three HLA-A*0201-restricted CTL epi-A*020 1-restricted CTL epitopes of SARS-CoVN protein. In addition epitopes of S protein (S978-986, S1203-1211, and S1167-1175), here we found two novel peptides S787-795 and S1042-1050 as S-specific CTL epitopes. Moreover, our identified N317-325 and S1042-1050 CTL epitopes could induce recall responses when IFN-gamma stimulation of blood CD8(+) T-cells revealed significant difference between normal healthy donors and SARS-recovered patients after those PBMCs were in vitro vaccinated with their cognate antigen. Our results would provide a new insight into the development of therapeutic vaccine in SARS. (c) 2006 Elsevier Inc. All rights reserved.
    關鍵詞: Biochemistry & Molecular Biology;Biophysics
    日期: 2006-05-26
    關聯: Biochemical and Biophysical Research Communications. 2006 May;344(1):63-71.
    Link to: http://dx.doi.org/10.1016/j.bbrc.2006.03.152
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0006-291X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000237408000011
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33646058584
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