國家衛生研究院 NHRI:Item 3990099045/13063
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/13063


    Title: Generation of high aspect-ratio pdms microfibers for 3D myelination culture of schwann cells
    Authors: Lin, HY;Chiu, IM;Wang, HD;Hsu, CH
    Contributors: Institute of Cellular and Systems Medicine;NHRI Graduate Student Program;Institute of Biomedical Engineering and Nanomedicine
    Abstract: We report a new approach to generate synthetic axons for in-vitro myelination culture of Schwann cells using high aspect-ratio (HAR) PDMS microfibers. In our approach, a microdevice containing ordered no-crossing suspended PDMS micropillars was first fabricated by using softlithography technique. Next, by applying a mechanical force to the device, the PDMS micropillars can be elongated to become microfibers > 100 um long with small diameters (< 20 μm). By changing the force, PDMS microfibers of different lengths and diameters may be obtained. Due to the biocompatible and optical transparency nature of PDMS, it is straight-forward to perform cell culture and microscopic analysis with the device. The microfiber device is also specially designed in a way that the myelin sheathes are easy to observe with a conventional microscope. We conducted S42 Schwann cell culture on the device and observed the cells form myelin-like cell morphology at the microfibers.
    Date: 2019-10
    Relation: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. 2019 Oct:288-289.
    Link to: https://microtas2019.org/
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85094943660
    Appears in Collections:[Ing-Ming Chiu] Periodical Articles
    [Others] Periodical Articles
    [Chia-Hsien Hsu] Periodical Articles

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