國家衛生研究院 NHRI:Item 3990099045/13009

NHRI > National Institute of Infectious Diseases and Vaccinology > Min-Shi Lee > Periodical Articles > Item 3990099045/13009

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/13009

Title:	Development and evaluation of vero cell-derived master donor viruses for influenza pandemic preparedness
Authors:	Chen, PL;Tzeng, TT;Hu, AYC;Wang, LHC;Lee, MS
Contributors:	National Institute of Infectious Diseases and Vaccinology
Abstract:	The embryonated egg-based platform currently produces the majority of seasonal influenza vaccines by employing a well-developed master donor virus (MDV, A/PR/8/34 (PR8)) to generate high-growth reassortants (HGRs) for A/H1N1 and A/H3N2 subtypes. Although the egg-based platform can supply enough seasonal influenza vaccines, it cannot meet surging demands during influenza pandemics. Therefore, multi-purpose platforms are desirable for pandemic preparedness. The Vero cell-based production platform is widely used for human vaccines and could be a potential multi-purpose platform for pandemic influenza vaccines. However, many wild-type and egg-derived influenza viruses cannot grow efficiently in Vero cells. Therefore, it is critical to develop Vero cell-derived high-growth MDVs for pandemic preparedness. In this study, we evaluated two in-house MDVs (Vero-15 and VB5) and two external MDVs (PR8 and PR8-HY) to generate Vero cell-derived HGRs for five avian influenza viruses (AIVs) with pandemic potentials (H5N1 clade 2.3.4, H5N1 clade 2.3.2.1, American-lineage H5N2, H7N9 first wave and H7N9 fifth wave). Overall, no single MDV could generate HGRs for all five AIVs, but this goal could be achieved by employing two in-house MDVs (vB5 and Vero-15). In immunization studies, mice received two doses of Vero cell-derived inactivated H5N1 and H7N9 whole virus antigens adjuvanted with alum and developed robust antibody responses.
Date:	2020-10-25
Relation:	Vaccines. 2020 Oct 25;8(4):Article number 626.
Link to:	http://dx.doi.org/10.3390/vaccines8040626
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2076-393X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000601797300001
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85094114635
Appears in Collections:	[Min-Shi Lee] Periodical Articles [Yung-Chih Hu] Periodical Articles [Tsai-Teng Tzeng] Periodical Articles

Files in This Item:

File	Description	Size	Format
SCP85094114635.pdf		3198Kb	Adobe PDF	381	View/Open

Loading...