國家衛生研究院 NHRI:Item 3990099045/12997
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 913388      線上人數 : 1134
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/12997


    題名: ANGPTL4 induces TMZ resistance of glioblastoma by promoting cancer stemness enrichment via the EGFR/AKT/4E-BP1 cascade
    作者: Tsai, YT;Ko, CY;Chang, WC;Hsu, TI
    貢獻者: NHRI Graduate Student Program
    摘要: Glioblastoma (GBM) is the most aggressive brain tumor, with a strong invasiveness and high tolerance to chemotherapy. With the current standard treatment combining temozolomide (TMZ) and radiotherapy, glioblastoma can be incurable due to drug resistance. The existence of glioma stem‐like cells (GSCs) is considered the major reason for drug resistance. However, the mechanism of GSC enrichment remains unclear. Herein, we found that the expression and secretion of angiopoietin‐like 4 protein (ANGPTL4) were obviously increased in GSCs. The overexpression of ANGPTL4 induced GSC enrichment that was characterized by BMI‐1 and SOX2 expression, resulting in TMZ resistance in GBM. Furthermore, epidermal growth factor (EGFR) phosphorylation induced 4E‐BP1 phosphorylation that was required for ANGPTL4‐induced GSC enrichment. In particular, ANGPTL4 induced 4E‐BP1 phosphorylation by activating PI3K/AKT and ERK cascades for inducing stemness. To elucidate the mechanism contributing to ANGPTL4 upregulation in GSCs, chromatin immunoprecipitation coupled with sequencing (ChIP‐Seq) revealed that specificity protein 4 (Sp4) associates with the promoter region, −979 to −606, and the luciferase reporter assay revealed that Sp4 positively regulates activity of the ANGPTL4 promoter. Moreover, both ANGPTL4 and Sp4 were highly expressed in GBM and resulted in a poor prognosis. Taken together, Sp4‐mediated ANGPTL4 upregulation induces GSC enrichment through the EGFR/AKT/4E‐BP1 cascade.
    日期: 2020-04
    關聯: FASEB Journal. 2020 Apr;34(S1):Abstract number 02019.
    Link to: http://dx.doi.org/10.1096/fasebj.2020.34.s1.02019
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0892-6638&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000546023101421
    顯示於類別:[其他] 會議論文/會議摘要

    文件中的檔案:

    沒有與此文件相關的檔案.



    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋