國家衛生研究院 NHRI:Item 3990099045/1285
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    题名: Bifunctional magnetic silica nanoparticles for highly efficient human stem cell labeling
    作者: Lu, CW;Hung, Y;Hsiao, JK;Yao, M;Chung, TH;Lin, YS;Wu, SH;Hsu, SC;Liu, HM;Mou, CY;Yang, CS;Huang, DM;Chen, YC
    贡献者: Center for Nanomedicine Research;Stem Cell Research Center
    摘要: A superparamagnetic iron oxide (SPIO) nanoparticle is emerging as an ideal probe for noninvasive cell tracking. However, its low intracellular labeling efficiency has limited the potential usage and has evoked great interest in developing new labeling strategies. We have developed fluorescein isothiocyanate (FITC)-incorporated silica-coated core-shell SPIO nanoparticles, SPIO@SiO2(FITC), with diameters of 50 nm, as a bifunctionally magnetic vector that can efficiently label human mesenchymal stem cells (hMSCs), via clathrin- and actin-dependent endocytosis with subsequent intracellular localization in late endosomes/lysosomes. The uptake process displays a time- and dose-dependent behavior. In our system, SPIO@SiO2(FITC) nanoparticles induce sufficient cell MRI contrast at an incubation dosage as low as 0.5 mu g of iron/mL of culture medium with 1.2 x 10(5) hMSCs, and the in vitro detection threshold of cell number is about 1 x 10(4) cells. Furthermore, 1.2 x 10(5) labeled cells can also be MRI-detected in a subcutaneous model in vivo. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes which can still be readily MRI detected. This is the first report that hMSCs can be efficiently labeled with MRI contrast nanoparticles and can be monitored in vitro and in vivo with a clinical 1.5-T MRI imager under low incubation concentration of iron oxide, short incubation time, and low detection cell numbers at the same time.
    关键词: Chemistry, Multidisciplinary;Nanoscience & Nanotechnology;Materials Science, Multidisciplinary
    日期: 2007-01
    關聯: Nano Letters. 2007 Jan;7(1):149-154.
    Link to: http://dx.doi.org/10.1021/nl0624263
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1530-6984&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000243381300026
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33846892018
    显示于类别:[楊重熙] 期刊論文
    [黃東明] 期刊論文
    [陳耀昌(2002-2004)] 期刊論文

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