國家衛生研究院 NHRI:Item 3990099045/12804
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 911564      在线人数 : 900
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/12804


    题名: Aptamer probed isolation of circulating tumor cells in cholangiocarcinoma patients
    作者: Gopinathan, P;Chiang, NJ;Wang, CH;Sinha, A;Tsai, YC;Tu, HC;Hung, SC;Hsu, PH;Shan, YS;Lee, GB
    贡献者: National Institute of Cancer Research
    摘要: Cholangiocarcinoma (CCA) is a typically lethal, highly metastatic cancer which poses diagnostic challenges and is consequently associated with poor prognosis and high metastatic capability. Since the currently used diagnostic biomarkers are limited in sensitivity and specificity, probes with high-affinity and good specificity are needed to study metastasis in CCA. Circulating tumor cells (CTCs) in blood have been widely accepted as an indicator of diseases progression and metastasis in various other cancers. We hereby report the first use of a CCA specific aptamer probe, coupled on magnetic beads for the isolation of CCA CTCs from 35 advanced stage/metastatic patients on an integrated microfluidic platform with 100 % efficiency (>1 CTC/mL were isolated). The isolated cells were identified via immunofluorescence staining (CK+CD45−Hoechst+) and cell counting was assisted by ImageJ. The binding target of the aptamer on the CCA cancer cells was further analyzed and determined to be desmoplakin by liquid chromatography-mass spectrometry, western blotting, and immunofluorescence staining. It is envisioned that this biosensing platform could therefore be used for proactive CCA diagnostics in the near future.
    日期: 2020-11-01
    關聯: Sensors and Actuators B: Chemical. 2020 Nov 1;322:Article number 128569.
    Link to: http://dx.doi.org/10.1016/j.snb.2020.128569
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0925-4005&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000573296500013
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85087808583
    显示于类别:[姜乃榕] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    SCP85087808583.pdf2144KbAdobe PDF343检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈