國家衛生研究院 NHRI:Item 3990099045/12793

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Title:	A study of drynaria fortunei in modulation of BMP-2 signalling by bone tissue engineering
Authors:	Dong, GC;Ma, TY;Li, CH;Chi, CY;Su, CM;Huang, CL;Wang, YH;Lee, TM
Contributors:	Institute of Biomedical Engineering and Nanomedicine
Abstract:	BACKGROUND/AIM: Drynaria Fortunei (Gusuibu; GSB) is a popular traditional Chinese medicine used for bone repair. An increasing number of studies have reported that GSB induces osteogenic differentiation in bone marrow mesenchymal stem cells (BMSCs). These results provide insight into the application of GSB for bone tissue engineering techniques used to repair large bone defects. However, few studies have described the molecular mechanisms of GSB. MATERIALS AND METHODS: In the present study, the effects of GSB and Naringin, a marker compound, on the binding of BMP-2 to BMPR and BMP-2-derived signal transduction were investigated using surface plasmon resonance (SPR) and co-culturing with BMPR-expressed cell line; C2C12, respectively. Furthermore, Naringin was also used to prepare Naringin-contained scaffolds for bone tissue engineering. The physical and chemical properties of these scaffolds were analyzed using scanning electron microscopy (SEM) and high-performance liquid chromatography (HPLC). These scaffolds were co-cultured with rabbit BMSCs in vitro and implanted into rabbit calvarial defects for bone repair assessment. RESULTS: The results showed that GSB and Naringin affect the binding of BMP and BMPR in SPR experiments. GSB is a subtle BMP modulator that simultaneously inhibits the binding of BMP-2 to BMPR-1A and enhances its binding to BMPR-1B. In contrast, Naringin inhibited BMP-2 binding to BMPR-1A. In vitro studies involving the phosphorylation of signals downstream of BMPR and SMAD showed that GSB and Naringin affected stem cell differentiation by inhibiting BMPR-1A signaling. When using GSB for bone tissue engineering, Naringin exhibited a higher capacity for slow and gradual release from the scaffold, which promotes bone formation via osteoinduction. Moreover, control and Naringin scaffolds were implanted into rabbit calvarial defects for 4 weeks, and Naringin enhanced bone regeneration in vivo significantly. CONCLUSION: GSB and its marker compound (Naringin) could inhibit the binding of BMP-2 and BMPR-1A to control cell differentiation by blocked BMPR-1A signaling and enhanced BMPR-1B signaling. GSB and Naringin could be good natural BMP regulators for bone tissue engineering.
Date:	2020-08
Relation:	Turkish Journal of Medical Sciences. 2020 Aug;50(5):1444-1453.
Link to:	http://dx.doi.org/10.3906/sag-2001-148
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1300-0144&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000566495700035
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85089850039
Appears in Collections:	[Guo-Chung Dong] Periodical Articles

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