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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/1279


    Title: Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking
    Authors: Huang, DM;Hung, Y;Ko, BS;Hsu, SC;Chen, WH;Chien, CL;Tsai, CP;Kuo, CT;Kang, JC;Yang, CS;Mou, CY;Chen, YC
    Contributors: Center for Nanomedicine Research;Stem Cell Research Center
    Abstract: Tracking the distribution of stem cells is crucial to their therapeutic use. However, the usage of current vectors in cellular labeling is restricted by their low internalizing efficiency. Here, we reported a cellular labeling approach with a novel vector composed of mesoporous silica nanoparticles (MSNs) conjugated with fluorescein isothiocyanate in human bone marrow mesenchymal stem cells and 3T3-L1 cells, and the mechanism about fluorescein isothiocyanate-conjugated MSNs (FITC-MSNs) internalization was studied. FITC-MSNs were efficiently internalized into mesenchymal stem cells and 3T3-L1 cells even in short-term incubation. The process displayed a time-and concentration-dependent manner and was dependent on clathrin-mediated endocytosis. In addition, clathrin-dependent endocytosis seemed to play a decisive role on more internalization and longer stay of FITC-MSNs in mesenchymal stem cells than in 3T3-L1 cells. The internalization of FITC-MSNs did not affect the cell viability, proliferation, immunophenotype,and differentiation potential of mesenchymal stem cells, and 3T3-L1 cells. Finally, FITC-MSNs could escape from endolysosomal vesicles and were retained the architectonic integrity after internalization. We conclude that the advantages of biocompatibility, durability, and higher efficiency in internalization suit MSNs to be a better vector for stem cell tracking than others currently used.
    Keywords: Biochemistry & Molecular Biology;Biology;Cell Biology
    Date: 2005-10
    Relation: FASEB Journal. 2005 Oct;19(12):2014-2016.
    Link to: http://dx.doi.org/10.1096/fj.05-4288fje
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0892-6638&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000232991100015
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=28744459447
    Appears in Collections:[黃東明] 期刊論文
    [楊重熙] 期刊論文
    [陳耀昌(2002-2004)] 期刊論文

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