國家衛生研究院 NHRI:Item 3990099045/12775

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Title:	The sex-specific association of phthalate exposure with DNA methylation and characteristics of body fat in children
Authors:	Chang, CH;Chen, CF;Tsai, YA;Wang, SL;Huang, PC;Chen, BH;Wu, MT;Chen, CC;Hsiung, CA;Chen, ML
Contributors:	National Institute of Environmental Health Sciences;Institute of Population Health Sciences
Abstract:	The present study assessed the association between phthalate exposure and mitochondrial DNA (mtDNA) polymerase γ (POLG) methylation along with the potential effect on the characteristics of body fat in children. A total of 152 children were enrolled. The urinary concentrations of phthalate metabolites were measured using ultraperformance liquid chromatography-tandem mass spectrometry. Genomic DNA was extracted from the buffy coat, and bisulfite-treated DNA was subjected to a pyrosequencing assay. In total, 17 CpG sites in the exon 2 region of POLG were included in the analysis. A multivariable regression model was applied to determine whether characteristics of body fat were associated with phthalate exposure and methylation of POLG. After adjustment for covariates, male children with a ten-fold increase in mono-methyl phthalate (MMP) or mono-benzyl phthalate (MBzP) concentrations had significantly higher measurements for total body fat (MMP: β = 6.47%; MBzP: β = 3.54%), and trunk fat (MMP: β = 6.67%; MBzP: β = 3.90%). Male children who had hypermethylation at the 2nd CpG site in exon 2 of POLG also had high measurements for BMI (β = 1.66 kg/m2), waist (β = 4.49 cm) and hip (β = 4.81 cm) circumference, total body fat (β = 5.48%), and trunk fat (β = 6.21%). A dose-response relationship existed between methylation at the 2nd CpG site in exon 2 of POLG and characteristics of body fat (p for trend<0.01). This study suggested that male children who are exposed to phthalic acid esters have high body weight, BMI, and body and trunk fat percentages. Methylation of the exon 2 region of POLG is a possible mechanism behind the causal effect of endocrine-disrupting substances.
Date:	2020-10
Relation:	Science of the Total Environment. 2020 Oct;737:Article number 139833.
Link to:	http://dx.doi.org/10.1016/j.scitotenv.2020.139833
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0048-9697&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000553713200004
Cited Times(Scopus):	https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85085912286
Appears in Collections:	[Po-Chin Huang] Periodical Articles [Shu-Li Wang] Periodical Articles [Chu-Chih Chen] Periodical Articles [Chao A. Hsiung] Periodical Articles

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